...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of MagPix Assays and Enzyme-Linked Immunosorbent Assay for Detection of Hemorrhagic Fever Viruses
【24h】

Comparison of MagPix Assays and Enzyme-Linked Immunosorbent Assay for Detection of Hemorrhagic Fever Viruses

机译：MagPix检测法和酶联免疫吸附法检测出血热病毒的比较

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detection. For decades, the gold-standard immunoassay for hemorrhagic fever detection has been the enzyme-linked immunosorbent assay (ELISA); however, newer technologies are emerging with increased sensitivities. One such technology is the Luminex MagPix platform using xMAP microspheres. Here, we compare the MagPix platform with a traditional ELISA for IgM and antigen detection of infections from Lassa and Ebola viruses (LASV and EBOV, respectively). For IgM detection in nonhuman primate samples, the MagPix platform was 5 and 25 times more sensitive in detecting LASV and EBOV, respectively, compared to that with ELISA. For antigen detection in buffer, the MagPix platform was 25 and 2.5 times more sensitive in detecting lower levels of LASV and EBOV, respectively. In both IgM and antigen detection assays, the MagPix platform demonstrated excellent reproducibility at the lower limit of detection (LLOD). These findings demonstrate that the MagPix platform is a viable diagnostic replacement for the ELISA for viral hemorrhagic fevers.
机译：病毒性出血热由于死亡率高，缺乏医疗对策以及它们有可能被用作生物恐怖主义手段，对世界的发达和发展中地区构成了重大威胁。预防这些疾病传播的关键是及早和准确的发现。几十年来，用于检测出血热的金标准免疫测定法一直是酶联免疫吸附测定法（ELISA）。但是，随着灵敏度的提高，出现了更新的技术。其中一种技术是使用xMAP微球的Luminex MagPix平台。在这里，我们将MagPix平台与用于IgM的传统ELISA以及从Lassa和埃博拉病毒（分别为LASV和EBOV）感染的抗原检测进行比较。对于非人类灵长类动物样本中的IgM检测，与使用ELISA相比，MagPix平台在检测LASV和EBOV方面的灵敏度分别高出5倍和25倍。对于缓冲液中的抗原检测，MagPix平台在检测较低水平的LASV和EBOV时的灵敏度分别提高了25倍和2.5倍。在IgM和抗原检测测定中，MagPix平台均在较低的检测下限（LLOD）下显示出出色的可重复性。这些发现表明，MagPix平台是病毒性出血热ELISA的可行诊断替代品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号