...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cell biology >LIGHT AND ELECTRON MICROSCOPE LOCALIZATION OF BINDING SITES OF ANTIBODIES AGAINST OVINE LUTEINIZING HORMONE AND ITS TWO SUBUNITS IN RAT ADENOHYPOPHYSIS USING PEROXIDASE-LABELED ANTIBODY TECHNIQUE
【24h】

LIGHT AND ELECTRON MICROSCOPE LOCALIZATION OF BINDING SITES OF ANTIBODIES AGAINST OVINE LUTEINIZING HORMONE AND ITS TWO SUBUNITS IN RAT ADENOHYPOPHYSIS USING PEROXIDASE-LABELED ANTIBODY TECHNIQUE

机译：应用过氧化物酶酶联抗体技术对大鼠腺病毒缺乏症大鼠卵黄体激素及其两个亚基的结合位点的光和电子显微镜定位

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The binding sites of antisera generated in the guinea pig against ovine luteinizing hormone (oLH) and its two subunits (oLHα and oLH?) have been localized in rat anterior pituitaries taken from normal or castrated males and from ovariectomized females with the peroxidase-labeled antibody method, using light and electron microscopy. With the light microscope, the cells positive with antiserum to ovine luteinizing hormone (A-oLH) were violet after the Alcian blue-periodic acid-Schiff (AB-PAS) staining; they were also positive for A-oLHα and for A-oLH? and, from castrated males, they displayed an increased affinity for A-oLH?. Another cell type which was blue after the AB-PAS method reacted with the A-oLHα only; these cells, presumably thyrotropic cells, were retracted after castration and, besides their affinity for A-oLHα, acquired an affinity for A-oLH?. As seen through the electron microscope, two cell types were positive for A-oLH, A-oLH?, and A-oLHα and may be identified as luteinizing hormone-secreting cells. Type A cells were characterized by two classes of rounded, secretory granules. Type B cells were smaller and contained only small secretory granules. 1 mo after the rats were castrated the type A cells were hypertrophied and vacuolized. In both cases the secretory granules were the main sites of the antigenicity with the three antisera. A positive reaction was also found in the cytoplasm, particularly in hypertrophied cells from ovariectomized females and with A-oLH?. The cisternae of the rough endoplasmic reticulum were usually negative, except in highly degranulated cells from ovariectomized females and with A-oLH?.
机译：豚鼠产生的抗血清黄体生成素（oLH）及其两个亚基（oLHα和oLH？）的结合位点位于大鼠前垂体中，该前垂体取自正常或去势雄性和经过氧化物酶标记的去卵巢雌性方法，使用光镜和电子显微镜。用光学显微镜观察到，对羊黄体生成激素（A-oLH）的抗血清呈阳性，经过阿尔辛蓝-高碘酸-席夫（AB-PAS）染色后呈紫红色。他们也对A-oLHα和A-oLH阳性？并且，从cast割的雄性中，它们显示出对A-oLHα的亲和力增加。在AB-PAS方法之后，另一种蓝色的细胞仅与A-oLHα反应。这些细胞，大概是促甲状腺细胞，在去势后被撤回，除了它们对A-oLHα的亲和力之外，还获得了对A-oLHα的亲和力。通过电子显微镜观察，A-oLH，A-oLHα和A-oLHα有两种细胞阳性，并且可以鉴定为促黄体素分泌的细胞。 A型细胞的特征是两类圆形的分泌颗粒。 B型细胞较小，仅含有小分泌颗粒。大鼠mo割后1个月，A型细胞肥大并空泡。在这两种情况下，分泌颗粒都是三种抗血清的抗原性主要部位。在细胞质中也发现了阳性反应，特别是在去卵巢的雌性和使用A-oLH 2的肥大细胞中。粗糙内质网的水箱通常是阴性的，除了在去卵巢的雌性和A-oLH2中高度脱颗粒的细胞中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号