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首页> 外文期刊>Journal of Clinical Microbiology >GyrB Polymorphisms Accurately Assign Invasive Viridans Group Streptococcal Species
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GyrB Polymorphisms Accurately Assign Invasive Viridans Group Streptococcal Species

机译：GyrB基因多态性准确地分配入侵的viridans组链球菌物种。

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Viridans group streptococci (VGS) are a heterogeneous group of medically important bacteria that cannot be accurately assigned to a particular species using conventional phenotypic methods. Although multilocus sequence analysis (MLSA) is considered the gold standard for VGS species-level identification, MLSA is not yet feasible in the clinical setting. Conversely, molecular methods, such as sodA and 16S rRNA gene sequencing, are clinically practical but not sufficiently accurate for VGS species-level identification. Here, we present data regarding the use of an ～400-nucleotide internal fragment of the gene encoding DNA gyrase subunit B (GyrB) for VGS species-level identification. MLSA, internal gyrB, sodA, full-length, and 5′ 16S gene sequences were used to characterize 102 unique VGS blood isolates collected from 2011 to 2012. When using the MLSA species assignment as a reference, full-length and 5′ partial 16S gene and sodA sequence analyses failed to correctly assign all strains to a species. Precise species determination was particularly problematic for Streptococcus mitis and Streptococcus oralis isolates. However, the internal gyrB fragment allowed for accurate species designations for all 102 strains. We validated these findings using 54 VGS strains for which MLSA, 16S gene, sodA, and gyrB data are available at the NCBI, showing that gyrB is superior to 16S gene and sodA sequence analyses for VGS species identification. We also observed that specific polymorphisms in the 133-amino acid sequence of the internal GyrB fragment can be used to identify invasive VGS species. Thus, the GyrB amino acid sequence may offer a more practical and accurate method for classifying invasive VGS strains to the species level.
机译：Viridans组链球菌（VGS）是医学上重要细菌的异质类，使用常规表型方法无法准确地将其分配给特定物种。尽管多基因座序列分析（MLSA）被认为是VGS物种水平鉴定的金标准，但MLSA在临床环境中尚不可行。相反，分子方法，例如 sodA 和16S rRNA基因测序，在临床上很实用，但对于VGS物种水平的鉴定还不够准确。在这里，我们提供有关使用编码DNA旋转酶亚基B（GyrB）的基因的〜400个核苷酸内部片段进行VGS物种水平鉴定的数据。 MLSA，内部 gyrB ， sodA ，全长和5'16S基因序列用于表征2011年至2012年收集的102种独特的VGS血液分离株。作为物种参考，全长和5'部分16S基因以及 sodA 序列分析未能正确地将所有菌株分配给一个物种。对于微生物链球菌和口头链球菌分离株而言，精确的物种确定尤其成问题。但是，内部的 gyrB 片段允许对所有102个菌株进行准确的物种命名。我们使用54个VGS菌株验证了这些发现，这些菌株的MLSA，16S基因， sodA 和 gyrB 数据在NCBI上可用，表明 gyrB 在VGS种类识别方面优于16S基因和 sodA 序列分析。我们还观察到内部GyrB片段的133个氨基酸序列中的特定多态性可用于识别侵入性VGS物种。因此，GyrB氨基酸序列可以为将侵入性VGS菌株分类到物种水平提供更实用和准确的方法。

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