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首页> 外文期刊>Journal of cell biology >The opsonizing ligand on Salmonella typhimurium influences incorporation of specific, but not azurophil, granule constituents into neutrophil phagosomes.
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The opsonizing ligand on Salmonella typhimurium influences incorporation of specific, but not azurophil, granule constituents into neutrophil phagosomes.

机译：鼠伤寒沙门氏菌上的调理配体影响将特定但非嗜蓝粒的颗粒成分掺入嗜中性粒细胞吞噬体。

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摘要

Phagosomes were purified from human neutrophils ingesting Salmonella typhimurium opsonized with adsorbed normal human serum or with rabbit IgG. Constituents within the phagosome were endogenously labeled by supplying the cells with 125INa during phagocytosis. Lactoferrin and vitamin B12 binding protein (TC1 and TC3), markers for specific granules, were present in the phagosomes from neutrophils ingesting S. typhimurium opsonized with IgG but were 3.5- to 5-fold less prominent in phagosomes from cells phagocytosing Salmonella bearing C3 fragments only. In contrast, iodinated azurophilic granule components, most prominently defensins, were the major constituents in phagosomes prepared under both opsonization conditions. Furthermore, labeled complement (CR1 and CR3) and immunoglobulin (Fc gamma RIII) receptors were incorporated in the phagosome regardless of the ligand mediating phagocytosis. These results suggest that the ligand-receptor interactions mediating phagocytosis influence incorporation of neutrophil-specific granule contents into phagosomes.
机译：从吞噬鼠伤寒沙门氏菌的人嗜中性粒细胞中纯化吞噬体，并用吸附的正常人血清或兔IgG调理。通过在吞噬过程中向细胞提供125INa内吞标记吞噬体内的成分。乳铁蛋白和维生素B12结合蛋白（TC1和TC3）是特定颗粒的标志物，存在于吞噬经IgG调理的鼠伤寒沙门氏菌的嗜中性粒细胞的吞噬物中，但在吞噬带有C3片段的沙门氏菌的细胞的吞噬物中的突出度降低了3.5至5倍。只要。相反，在两种调理条件下制备的吞噬体中，碘化的嗜酸性颗粒成分（最突出的防御素）是主要成分。此外，无论配体介导吞噬作用如何，都将标记的补体（CR1和CR3）和免疫球蛋白（FcγRIII）受体掺入吞噬体中。这些结果表明介导吞噬作用的配体-受体相互作用影响嗜中性粒细胞特异性颗粒内容物向吞噬体的掺入。

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