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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Comparison of Mast Cells and Inflammatory Cells within Periapical Lesions and Comparison of Degranulated Mast Cells Between Fibrous andInflamed Area in Radicular Cysts:An Immunohistochemical Study
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Comparison of Mast Cells and Inflammatory Cells within Periapical Lesions and Comparison of Degranulated Mast Cells Between Fibrous andInflamed Area in Radicular Cysts:An Immunohistochemical Study

机译：根尖囊肿内根治性病变肥大细胞与炎性细胞的比较及纤维组织与发炎区域肥大细胞的肉芽组织的比较：免疫组织化学研究

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Objective: The role of mast cells as the key effector of allergic inflammation, anaphylactic inflammatory reactions and in the pathogenesis of chronic inflammation, is well-known. The present study is adopted to compare mast cells and inflammatory cells within periapical granuloma and cysts and localize the mast cells and quantify their number in the periapical cysts so as to propose a role of mast cells in the pathogenesis of this lesion.Materials and Methods: Biopsy specimens of 30 periapical lesions were stained with hematoxylin?eosin, and immuno - histochemical Mast Cell Tryptase from Bio SB (IHC detection system kit) antibody. The tryptase positive mast cells and mono - nuclear inflammatory cells were counted in 10 consecutive high power fields (100X) using the binocular microscope from Motic attached to a computer with Motic Advanced Images 3.2 software.Results: Comparative microscopic analysis indicated that periapical cyst shows more percentage of mast cells and less percentage of inflammatory cell than periapical granuloma (comparison of mean and standard deviation of total number of mast cells and inflammatory cells, mast cells 3.15±1.39 in the granuloma group and 4.43±1.91in the cyst group, inflammatory cells, 67.11±1.2 in the granuloma group and 52.66±0.8 in the cyst group). Numerous degranulated mast cells were observed in the fibrous wall than the inflammatory infiltrate of the periapical cysts. The mean and standard deviation of degranulated mast cells between the inflammatory and fibrous zone within the cyst group, being 0.95±1.10 and1.68±1.34 respectively. The values varied significantly between the two zones.Conclusion: The number of inflammatory cells in the cyst group is less than periapical granuloma and total number of mast cells in the cyst group is more as compared to periapical granuloma. The degranulated cells were quantified and they were higher in the fibrous area of the cysts than the inflammatory zone. This study could support the fact that the various mediators released on degranulation play a role in the connective tissue remodeling, chronicity and expansion of the periapical lesion.
机译：目的：肥大细胞作为变应性炎症，过敏性炎症反应以及慢性炎症的发病机理的关键作用是众所周知的。本研究旨在比较根尖周肉芽肿和囊肿中的肥大细胞和炎性细胞，并定位肥大细胞并量化其在根尖周囊肿中的数量，从而提出肥大细胞在该病变的发病机理中的作用。用苏木精-伊红和来自Bio SB的免疫组织化学肥大细胞类胰蛋白酶（IHC检测系统试剂盒）抗体对30个根尖周病变的活检标本进行染色。使用装有Motic Advanced Images 3.2软件的计算机连接到Motic的双目显微镜，在10个连续的高倍视野（100X）中对类胰蛋白酶阳性的肥大细胞和单核炎性细胞进行计数。结果：对比显微镜分析表明，根尖周囊肿显示出更多肥大细胞百分比和炎性细胞百分比低于根尖肉芽肿（肥大细胞和炎性细胞总数的均值和标准差的比较，肉芽肿组的肥大细胞为3.15±1.39，囊肿组的肥大细胞为4.43±1.91，炎症细胞，肉芽肿组为67.11±1.2，囊肿组为52.66±0.8）。在纤维壁中观察到许多脱粒的肥大细胞，而不是根尖周囊肿的炎性浸润。囊肿组内炎症区和纤维区之间的肥大细胞脱颗粒平均数和标准差分别为0.95±1.10和1.68±1.34。结论：囊肿组炎性细胞数量少于根尖周肉芽肿，肥大细胞总数比根尖周肉芽肿多。对脱粒的细胞进行定量，并且它们在囊肿的纤维区域中比炎症区域高。这项研究可以支持以下事实：脱颗粒释放的各种介体在结缔组织重塑，慢性和根尖周病变的扩展中起作用。

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