...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Phenotypic Detection and Rate of Nasal Carriage of Heterotypic Borderline Oxacillin Resistant Staphylococcus aureus In Pre-clinical Medical Students from Malaysia
【24h】

Phenotypic Detection and Rate of Nasal Carriage of Heterotypic Borderline Oxacillin Resistant Staphylococcus aureus In Pre-clinical Medical Students from Malaysia

机译：来自马来西亚的临床前医学生对异型边界线抗奥沙西林金黄色葡萄球菌的表型检测和鼻腔通气率

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: We investigated the rate of nasal colonization of heterogeneous Staphylococcus aureus (Methicillin Resistant S. aureus (MRSA), borderline oxacillin resistant S. aureus (BORSA), as well as hetero-resistant patterns exhibited by S.aureus, in 157 pre-clinical medical students from Melaka Manipal Medical College, Manipal University, in order to determine the carrier profile among the student community from Malaysia before they entered the clinics during the phase I stage II of their medical program in India. Methods: Oxacillin agar screen technique was employed to screen for MRSA. Broth macrodilution method was used to detect Minimum inhibitory concentration (MIC)values ranging between 0.25-16μg/ml. MHA broth incorporated with oxacillin antibiotic was diluted from 0.25μg/ml, 0.5μg/ml, 1μg/ml, 2μg/ml, 4μg/ml, 8μg/ml to 16μg/ml. The Cefoxitin disc diffusion test was done in order to check if BORSA isolated were mecA negative. Results: Out of the 37 coagulase positive Staphylococcus aureus samples screened, 27 (72.97%) samples showed an MIC range between 0.25μg/ml-1.0μg/ml. Ten (27.02%) samples had the MIC range between 2μg/ml-8μg/ml; out of which 3 (8.10%) samples had an MIC value of 2μg/ml, and 3 (8.10%) samples showed MIC as 4μg/ml, while 4 (10.81%) showed the MIC to be 8μg/ml in broth dilutions. None (0%) showed MIC for 16μg/ml, and the Cefoxitin disk diffusion tests conducted showed that the 37 isolates of CoPS had zones of inhibition >21mm in diameter. Conclusions: This study revealed the asymptomatic nasal carriage of BORSA (6.49%) and coagulase positive methicillin susceptible (17.53%) Staphylococcus aureus(MSSA) in healthy student volunteers. Ten (27.02%) samples that had MIC values between 2-8μg/ml showed cefoxitin susceptibility, proving the absence of the mecA gene; they were classified as BORSA; while 27 (72.97%) samples that were cefoxitin susceptible mecA negative strains showed MIC values that ranged between 0.25 μg/ml to 1.0 μg/ml, and were classified as MSSA.
机译：背景：我们调查了157年前的异种金黄色葡萄球菌（耐甲氧西林金黄色葡萄球菌（MRSA），耐奥沙西林的金黄色葡萄球菌（BORSA）的鼻腔定植率以及金黄色葡萄球菌表现出的异种耐药模式。来自印度马尼帕尔大学马六甲马尼帕尔医学院的临床医学生，目的是确定他们在印度医疗计划的第一阶段II期进入诊所之前，来自马来西亚学生社区的携带者概况。用MRSA进行筛选，采用肉汤微量稀释法检测最低抑菌浓度（MIC）值，范围在0.25-16μg/ ml之间;将结合奥沙西林抗生素的MHA肉汤稀释至0.25μg/ ml，0.5μg/ ml，1μg/ ml结果：在37个凝固酶阳性葡萄球菌中，分别用2μg/ ml，4μg/ ml，8μg/ ml至16μg/ ml进行头孢西丁圆片扩散试验，以检查分离出的BORSA是否为mecA阴性。我们筛选出的金黄色葡萄球样品有27个（72.97％）样品的MIC范围在0.25μg/ml-1.0μg/ ml之间。十个样品（27.02％）的MIC范围在2μg/ml-8μg/ ml之间。其中3个（8.10％）样品的MIC值为2μg/ ml，3个（8.10％）样品的MIC为4μg/ ml，而4个（10.81％）的肉汤稀释液中的MIC为8μg/ ml。没有（0％）的MIC为16μg/ ml，头孢西丁圆盘扩散试验表明37株CoPS菌株的抑菌圈直径大于21mm。结论：这项研究揭示了健康学生志愿者中无症状的鼻腔鼻支架BORSA（6.49％）和易感凝固酶阳性的甲氧西林（17.53％）金黄色葡萄球菌（MSSA）。十个（27.02％）MIC值在2-8μg/ ml之间的样品显示出头孢西丁敏感性，这证明不存在mecA基因。他们被归类为BORSA;头孢西丁易感mecA阴性菌株的27个样本（72.97％）的MIC值介于0.25μg/ ml至1.0μg/ ml之间，被分类为MSSA。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号