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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Auto Fluorescence And Fourier Transform ? Infra Red (FTIR) Spectral Investigation On Di Ethyl Nitrosamine (DEN) Induced Hepatocellular Carcinoma, Treated With Pericarp Extract Of Garcinia Mangostana Linn In Rats
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Auto Fluorescence And Fourier Transform ? Infra Red (FTIR) Spectral Investigation On Di Ethyl Nitrosamine (DEN) Induced Hepatocellular Carcinoma, Treated With Pericarp Extract Of Garcinia Mangostana Linn In Rats

机译：自发荧光和傅立叶变换？藤黄果皮提取物对大鼠二乙基亚硝胺（DEN）诱导的肝细胞癌的红外（FTIR）光谱研究

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The aim of the present study was to analyze the tumour activity of diethyl nitrosamine (DEN); a chemical carcinogen which induced hepatocellular carcinoma in the liver tissue samples of male albino rats. The analysis has been carried out in the following conditions; normal, tumour induced and tumour treated liver tissue samples. In all these cases, the action of the diethyl nitrosamine drug in inducing tumours in the liver has been investigated. Garcinia mangostana pericarp extract was the Ayurvedic drug which was chosen for the treatment of hepatocellular carcinoma. The control liver tissues, those in which tumour formation was induced by using diethyl nitrosamine and those in which the tumour was treated with the Garcinia mangostana pericarp extract after inducing tumor formation were analyzed by using auto fluorescence spectroscopy and fourier transform infra red (FTIR) spectroscopy. The qualitative spectral analysis was done by using auto fluorescence spectroscopy and the quantitative study was carried out by using FTIR spectroscopy. The tissue samples were experimented with auto fluorescence spectroscopy at the excitation wavelengths of 280nm, 325nm and 405nm, which exhibits emission due to tryptophan, collagen and porphyrin, respectively. It was observed that in all the tissue samples, the effect of diethylnitrosamine in inducing tumours was predominant. However, on treating the tumours with the pericarp extract of Garcinia mangostana, they responded positively. FTIR spectra have been recorded in the mid frequency region of 4000 ? 450 cm-1 for all the liver tissue samples. The samples were analyzed quantitatively by using intensity ratio calculation among the selected absorption peaks to study the biochemical changes in the tissue samples. From this study, it was found that the total protein content was decreased in the liver tissues after inducing them with diethyl nitrosamine, thus causing the hepato cellular carcinoma. During the recovery phase, the decreased levels of the bio-chemical constituents were restored to near normal levels.
机译：本研究的目的是分析二乙基亚硝胺（DEN）的肿瘤活性。一种化学致癌物，可在雄性白化病大鼠的肝组织样品中诱发肝细胞癌。分析是在以下条件下进行的;正常，肿瘤诱导和肿瘤治疗的肝组织样本。在所有这些情况下，已经研究了二乙基亚硝胺药物在肝脏中诱导肿瘤的作用。芒果藤果皮提取物是阿育吠陀药物，被选择用于治疗肝细胞癌。使用自动荧光光谱和傅里叶变换红外光谱（FTIR）对对照肝脏组织，使用二乙基亚硝胺诱导肿瘤形成的组织以及用诱导的肿瘤形成后用藤黄果皮提取物治疗的组织进行分析。 。使用自动荧光光谱仪进行定性光谱分析，并使用FTIR光谱仪进行定量研究。用自动荧光光谱在280nm，325nm和405nm的激发波长下对组织样品进行实验，其分别由于色氨酸，胶原蛋白和卟啉而显示出发射。观察到在所有组织样品中，二乙基亚硝胺在诱导肿瘤中的作用是主要的。然而，在用藤黄果皮提取物治疗肿瘤时，它们反应良好。 FTIR光谱已记录在4000？所有肝脏组织样本为450 cm-1。通过使用选定吸收峰之间的强度比计算对样品进行定量分析，以研究组织样品中的生化变化。从该研究中发现，用二乙基亚硝胺诱导肝脏组织后，总蛋白质含量降低，从而引起肝细胞癌。在恢复阶段，生化成分下降的水平恢复到接近正常水平。

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