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首页> 外文期刊>Journal of cell biology >Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase
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Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase

机译：信号蛋白磷脂酶D通过纤毛的循环仅要求BBSome用于出口阶段

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摘要

The BBSome is a complex of seven proteins, including BBS4, that is cycled through cilia by intraflagellar transport (IFT). Previous work has shown that the membrane-associated signaling protein phospholipase D (PLD) accumulates abnormally in cilia of Chlamydomonas reinhardtii bbs mutants. Here we show that PLD is a component of wild-type cilia but is enriched ～150-fold in bbs4 cilia; this accumulation occurs progressively over time and results in altered ciliary lipid composition. When wild-type BBSomes were introduced into bbs cells, PLD was rapidly removed from the mutant cilia, indicating the presence of an efficient BBSome-dependent mechanism for exporting ciliary PLD. This export requires retrograde IFT. Importantly, entry of PLD into cilia is BBSome and IFT independent. Therefore, the BBSome is required only for the export phase of a process that continuously cycles PLD through cilia. Another protein, carbonic anhydrase 6, is initially imported normally into bbs4 cilia but lost with time, suggesting that its loss is a secondary effect of BBSome deficiency.
机译：BBSome是七种蛋白质（包括BBS4）的复合体，通过鞭毛内转运（IFT）在纤毛中循环。先前的工作表明，膜相关信号蛋白磷脂酶D（PLD）在莱茵衣藻bbs突变体的纤毛中异常积累。在这里，我们显示PLD是野生型纤毛的一个成分，但在bbs4纤毛中却富集了约150倍。随着时间的流逝，这种积累逐渐发生，并导致睫状脂质成分改变。将野生型BBSomes导入bbs细胞后，PLD迅速从突变纤毛中去除，表明存在有效的BBSome依赖性机制来输出睫状PLD。此出口需要逆行IFT。重要的是，PLD进入纤毛的过程与BBSome和IFT无关。因此，仅在连续循环通过纤毛循环PLD的过程的输出阶段才需要BBSome。另一种蛋白质碳酸酐酶6最初通常会导入bbs4纤毛中，但会随着时间的流逝而丢失，这表明其丢失是BBSome缺乏症的次要作用。

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