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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Evaluation of the Virulence Markers in the Clinical Isolates of Citrobacter Species: The First Report from India
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Evaluation of the Virulence Markers in the Clinical Isolates of Citrobacter Species: The First Report from India

机译：柠檬酸杆菌属临床分离物中毒力标记的评估：来自印度的第一份报告

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Background: Citrobacter, a gram negative bacillus, is emerging as one of the major pathogens in hospital settings, with a potential to cause serious infections. Virulence markers are the factors which enhance the survival ability of the micro-organisms. Among the gram negative bacilli, these have been extensively studied in Escherichia coli. To the best of our knowledge as per internet search, we report that this study has been done for the first time in India on Citrobacter.Aims: An investigation was done to determine the ability of Citrobacter in producing virulence markers like the ability to survive the serum bactericidal activity, the resistance to the intracellular killing in the Polymorphonuclear Leucocytes (PMNLs) and the Cell Surface Hydrophobicity (CSH).Settings and Design: This prospective study was conducted in the Department of Microbiology of a tertiary care hospital in Aligarh, India, for a period of one year and six months.Material and Methods: A total of 105 clinical isolates and 20 control isolates from healthy subjects were identified as having Citrobacter spp. by using standard biochemical tests and they were studied for the presence of virulence markers like the ability to survive the serum bactericidal activity, the resistance to the intracellular killing in the Polymorphonuclear Leucocytes (PMNLs) and the cell surface hydrophobicity. The statistical analysis which was used: The standard deviations and the p-values were calculated for comparison.Results: Out of the 105 clinical isolates of Citrobacter which were studied, 86.6% isolates were resistant to 100% serum after 180 minutes of incubation and intracellular killing in PMNL was demonstrated in 17% strains. The Salt Aggregation Test (SAT) for the cell surface hydrophobicity was positive in 17.1% strains at different concentrations of ammonium sulphate. The presence of more than one virulence marker was present in 71.4% of the clinical isolates while in the control isolates.Conclusions: The presence of the virulence markers in Citrobacter demonstrated its pathogenic potential. Its invasiveness and ability to disseminate can be studied by identifying these markers.
机译：背景：柠檬酸杆菌（一种革兰氏阴性杆菌）正在成为医院环境中的主要病原体之一，有可能引起严重感染。毒力标记是增强微生物存活能力的因素。在革兰氏阴性杆菌中，这些已在大肠杆菌中进行了广泛研究。据互联网搜索所知，我们报告该研究是首次在印度进行的关于柠檬酸杆菌的研究。目的：进行了一项调查以确定柠檬酸杆菌产生毒力标记物的能力，例如存活的能力。血清杀菌活性，对多形核白细胞（PMNLs）细胞内杀伤的抵抗力和细胞表面疏水性（CSH）。环境与设计：这项前瞻性研究是在印度阿里加尔市一家三级护理医院的微生物学部门进行的，材料和方法：从健康受试者中共鉴定出105株临床分离株和20株对照分离株为柠檬酸杆菌。通过使用标准的生化测试，我们研究了其毒性标志物的存在，例如血清杀菌活性的生存能力，多形核白细胞（PMNLs）对细胞内杀伤的抵抗力和细胞表面疏水性。结果：在所研究的105种柠檬酸杆菌临床分离株中，有86.6％的分离株在孵育180分钟和细胞内后对100％的血清具有抗性。在17％的菌株中证明了PMNL的杀灭作用。在不同浓度的硫酸铵下，盐聚集试验（SAT）在17.1％的菌株中对细胞表面疏水性呈阳性。在临床分离株中有71.4％存在一种以上的毒性标记，而在对照菌株中存在。结论：柠檬酸杆菌中的毒性标记表明了其致病潜力。可以通过鉴定这些标记物来研究其侵袭性和传播能力。

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