...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Comparative Study of Genotoxicity in Different Tobacco Related Habits using Micronucleus Assay in Exfoliated Buccal Epithelial Cells
【24h】

Comparative Study of Genotoxicity in Different Tobacco Related Habits using Micronucleus Assay in Exfoliated Buccal Epithelial Cells

机译：使用脱落核颊上皮细胞中的微核试验比较不同烟草相关习性的遗传毒性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Oral cancer is one of the most debilitating diseases afflicting mankind. Consumption of tobacco in various forms constitutes one of the most important etiological factors in initiation of oral cancer. When the focus of today?s research is to determine early genotoxic changes in human cells, micronucleus (MN) assay provides a simple, yet reliable indicator of genotoxic damage.Aims and Objectives: To identify and quantify micronuclei in the exfoliated cells of oral mucosa in individuals with different tobacco related habits and control group, to compare the genotoxicity of different tobacco related habits between each group and also with that of control group.Patients and Methods: In the present study buccal smears of 135 individuals with different tobacco related habits & buccal smears of 45 age and sex matched controls were obtained, stained using Giemsa stain and then observed under 100X magnification in order to identify and quantify micronuclei in the exfoliated cells of oral mucosa.Results: The mean Micronucleus (MN) count in individuals having smoking habit were 3.11 while the count was 0.50, 2.13, and 1.67 in normal control, smoking with beetle quid and smokeless tobacco habit respectively. MN count in smokers group was 2.6 times more compared to normal controls. MN count was more even in other groups when compared to normal control but to a lesser extent.Conclusion: From our study we concluded that tobacco in any form is genotoxic especially smokers are of higher risk and micronucleus assay can be used as a simple yet reliable marker for genotoxic evaluation.
机译：背景：口腔癌是困扰人类的最衰弱疾病之一。各种形式的烟草消费是引发口腔癌的最重要的病因之一。当今天的研究重点是确定人类细胞中的早期遗传毒性变化时，微核（MN）分析提供了一种简单而可靠的遗传毒性损害指标。目的和目的：鉴定和定量口腔黏膜脱落细胞中的微核在具有不同烟草相关习惯和对照组的个体中，比较各组之间以及与对照组之间不同烟草相关习惯的遗传毒性。患者和方法：在本研究中，我们对135名具有不同烟草相关习惯的个体进行了口腔涂片检查，获得了45个年龄和性别相匹配的对照的颊涂片，使用Giemsa染色进行染色，然后在100倍放大倍率下观察，以鉴定和定量口腔黏膜脱落细胞中的微核。结果：吸烟个体的平均微核（MN）计数正常对照组的吸烟习惯为3.11，而正常对照的计数为0.50、2.13和1.67，吸烟者有甲虫和无烟烟草习惯 分别。吸烟者组的MN计数是正常对照组的2.6倍。结论：根据我们的研究，我们得出结论，任何形式的烟草均具有遗传毒性，尤其是吸烟者具有更高的风险，微核化验可作为一种简单而可靠的方法，结论是：在其他人群中，MN计数甚至更高。遗传毒性评估的标记。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号