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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Accessory Gene Regulator Types of Staphylococcus aureus Isolated in Gorgan, North of Iran
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Accessory Gene Regulator Types of Staphylococcus aureus Isolated in Gorgan, North of Iran

机译：伊朗北部戈尔恩分离的金黄色葡萄球菌的辅助基因调控子类型

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Background:Staphylococcus aureus is a gram-positive bacterium that has remained a persistent pathogen, causing infections such as endocarditis, meningitis, and toxic shock syndrome in humans. The accessory gene regulator (agr) system of Staphylococcus aureus is responsible for controlling the expression of many genes that code for virulence factors. In this study, we assessed the S.aureus agr Group, based on their source of isolation, in Gorgan, North of Iran. Materials and Methods:DNA of 194 S. aureus isolates was extracted by lysozyme-phenol chloroform method, which included 85 clinical samples, 58 samples which were isolated from noses of health care workers and 51 cases which were obtained from food products in Gorgan, northern Iran. PCR-based assays were used to evaluate agr locus nucleotide polymorphism for the identification of agr speci fi city Group. Distributions of each agr Group were determined and comparison between different sources was assessed by X 2 . A p-value of <0.05 was considered as signi fi cant. Results:The majority of isolates belonged to agr Group I (43.3%), followed by agr Group III (28.87%), agr Group II (22.68%), and agr Group IV (5.15%). In our study, a majority of S. aureus isolates were recovered from health care workers and food product specimens were of agr Group I and isolates which were recovered from patients were of agr Group III. These differences were statistically signi fi cant (P=0.005). There was no statistical difference between the source of isolation of clinical samples of S.aureus and agr type.Conclusion:Agr Group I was predominant among health care workers and food product specimens in Gorgan, North of Iran, but in strains which were isolated from patients, agr Group III was predominant. Investigating the possible role of agr Group III in Staphylococcus aureus infection in future studies is recommended.
机译：背景：金黄色葡萄球菌是一种革兰氏阳性细菌，一直是一种持久的病原体，可引起人类感染，如心内膜炎，脑膜炎和中毒性休克综合症。金黄色葡萄球菌的辅助基因调节剂（agr）系统负责控制许多编码毒力因子的基因的表达。在这项研究中，我们根据分离的来源评估了伊朗北部格根的金黄色葡萄球菌农业集团。材料与方法：用溶菌酶-苯酚氯仿法提取194株金黄色葡萄球菌的DNA，包括临床样本85份，从医护人员的鼻子中提取58份样本，以及从北部戈尔甘食品提取的51例。伊朗。基于PCR的分析用于评估农业基因座核苷酸多态性，以鉴定农业特异性城市群。确定每个农业集团的分布，并通过X 2评估不同来源之间的比较。 P值<0.05被认为是重要的。结果：大多数分离物属于agr组I（43.3 ％），其次是agr组III（28.87 ％），agr组II（22.68 ％）和agr组IV（5.15 ％） 。在我们的研究中，大多数金黄色葡萄球菌分离物是从医护人员中回收的，食品标本属于agr组I，而从患者中回收的分离物则属于agr组III。这些差异具有统计学意义（P = 0.005）。结论：金黄色葡萄球菌临床样本的分离来源与agr类型之间无统计学差异。结论：在伊朗北部戈尔甘的卫生保健工作者和食品样本中，Agr组占主导地位，但从病人中，agr组为主要。建议在以后的研究中研究agr组III在金黄色葡萄球菌感染中的可能作用。

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