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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of HumanSpermatozoa Function
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Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of HumanSpermatozoa Function

机译：精浆的pH值，无机磷酸盐，总钙和电离钙浓度在评估人类精子功能中的作用

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Introduction: Fertilization in humans is dependent on viability of the male spermatozoa among other factors and there have been conflicting reports on the role of pH, calcium and phosphate concentrations in sperm function. This study therefore aimed to investigate seminal plasma pH, inorganic phosphate, total and ionized calcium concentrations relative to spermatozoa function. Material and Methods: Seminal plasma concentrations of pH, total calcium, ionized calcium (Ca++); inorganic phosphate, motility and spermatozoa count were determined in 80 males by standard methods. Results: Forty-nine of the subjects had normal spermatozoa motility (> 60%) and 31 had hypomotility (< 60%). The hypomotility group exhibited lower calcium ion (Ca2 +) concentrations; 0.19+0.01mmol/L compared with normal motility group; 0.24+0.01mmol/L (p<0.001) the latter also had significantly higher inorganic phosphate; 7.83+1.27 while the former had 5.64+1.62mmol/L (p= 0.004). The mean spermatozoa counts for hypomotility and normal motility group were 42.0 ± 13 x 106 , 72.35 + 20 x 106 respectively (p< 0.001). No significant differences were observed in pH, volume of ejaculate and total calcium concentration between the hypomotility and normal motility groups The mean concentrations of pH were 7.51 ± 0.02 and 7.54 ± 0.03 respectively (p= 0.21) and total calcium; 3.10 ± 0.12 and 3.36 ± 0.14mmol/L respectively (p= 0.16 ). There was a significant difference in percentage of abnormal forms in both groups with hypomotile group having 36% compared to mormal motility group with 5% (p< 0.05).Conclusion: Correlations were observed between seminal concentrations of calcium ions, inorganic phosphate, spermatozoa count and motility but not with total calcium concentrations and pH and therefore should be considered in understanding male infertility and preparation of media for sperm preservation for in vitro fertilization.
机译：简介：人类受精取决于男性精子的生存能力以及其他因素，关于pH，钙和磷酸盐浓度在精子功能中的作用的报道相互矛盾。因此，本研究旨在研究精浆的pH值，无机磷酸盐，总和离子钙浓度与精子功能的关系。材料和方法：精浆的pH值，总钙，离子钙（Ca ++）;通过标准方法测定了80名男性的无机磷酸盐，活力和精子计数。结果：四十九名受试者的精子活动力正常（> 60％），31名运动能力低下（<60％）。低动力组表现出较低的钙离子（Ca 2+）浓度。与正常运动组相比，为0.19 + 0.01mmol / L; 0.24 + 0.01mmol / L（p <0.001），后者的无机磷酸盐也明显较高; 7.83 + 1.27，而前者为5.64 + 1.62mmol / L（p = 0.004）。运动力减退组和正常运动力组的平均精子数分别为42.0±13 x 106、72.35 + 20 x 106（p <0.001）。低动力组和正常运动组之间的pH值，射精量和总钙浓度无明显差异。pH的平均浓度分别为7.51±0.02和7.54±0.03（p = 0.21）和总钙。分别为3.10±0.12和3.36±0.14mmol / L（p = 0.16）。动力低下组为36％的两组中异常形式的百分比与动能组为5％的异常组的百分比有显着差异（p <0.05）。磷酸盐，精子数量和活力，但不与总钙浓度和pH值有关，因此在理解男性不育症和准备用于体外受精的精子保存培养基时应考虑。

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