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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Comparative Evaluation of ThreeHerbal Solutions on the Disinfection ofGuttapercha Cones: An In vitro Study ZC21-ZC24
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Comparative Evaluation of ThreeHerbal Solutions on the Disinfection ofGuttapercha Cones: An In vitro Study ZC21-ZC24

机译：ZH21-ZC24体外研究三种草药溶液消毒牙龈锥的比较研究

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Introduction: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. Aim: The aim of the study was to evaluate rapid, reliable, convenient and effective method of disinfection of GP cones using readily available herbal solutions such as Aloevera Juice, Amla Juice and Pancha Tulsi. Materials and Methods: Ninety GP cones were selected for the study. Based on the contaminants used, 80 GP cones were divided into two groups: Group A and Group B with 40 cones in each group and the remaining 10 cones which were uncontaminated served as negative control. The cones from Group A were contaminated with Staphylococcus aureus (S. aureus) and Group B were contaminated with Enterococcus faecalis (E. faecalis). The contaminated cones from both groups were subdivided into four groups with 10 cones in each group. Group I cones were disinfected with Aloevera Juice, Group II with Amla Juice and Group III with Pancha Tulsi. The fourth group consisted of GP cones without any disinfection which served as positive control. All the cones were then incubated in thioglycollate media for seven days. The thioglycollate media was subcultured and colony forming units were counted. The data were analyzed by one way ANOVA (analysis of variance) and Post-hoc Tukey test using SPSS 17.0 software. Results: Mean colony forming units were compared in all the groups and there was a statistically significant difference present among the groups (p<0.01). Pancha Tulsi was found to be most effective disinfectant followed by Amla Juice. Conclusion: All the herbal solutions were found to be effective in the disinfection of GP points. However, Pancha Tulsi possesses superior antibacterial activity when compared with Aloevera Juice and Amla juice.
机译：简介：Guttapercha（GP）锥通常由制造商以无菌，密封包装提供，但一旦暴露在牙科诊所环境中或什至通过处理即可;它们可能被多种微生物污染。 GP锥的补充净化至关重要。由于不能通过湿热或干热进行消毒，因此应使用消毒剂进行冷消毒。目的：该研究的目的是评估使用现成的草药溶液（如Aloevera果汁，Amla果汁和Pancha Tulsi）对GP锥消毒的快速，可靠，方便和有效的方法。材料和方法：选择90个GP锥进行研究。根据使用的污染物，将80个GP锥体分为两组：A组和B组，每组40个锥体，其余10个未污染的锥体用作阴性对照。来自A组的视锥细胞被金黄色葡萄球菌（S. aureus）污染，而B组被粪肠球菌（E. faecalis）污染。两组的污染锥均分为四组，每组10个。第一组使用Aloevera果汁消毒，第二组使用Amla果汁消毒，第三组使用Pancha Tulsi消毒。第四组由未经任何消毒的GP锥组成，用作阳性对照。然后将所有视锥细胞在巯基乙酸盐培养基中孵育7天。将巯基乙酸盐培养基传代培养并计数菌落形成单位。使用SPSS 17.0软件通过单因素方差分析（方差分析）和事后Tukey检验分析数据。结果：比较所有组的平均菌落形成单位，各组之间存在统计学差异（p <0.01）。 Pancha Tulsi被发现是最有效的消毒剂，其次是Amla Juice。结论：所有草药溶液均被发现对GP穴位消毒有效。但是，与Aloevera果汁和Amla果汁相比，Pancha Tulsi具有卓越的抗菌活性。

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