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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases
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Cell Culture, Technology: Enhancing the Culture of Diagnosing Human Diseases

机译：细胞培养技术：增强人类疾病诊断文化

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Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in vitro). Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic viral isolation depends on the availability of permissible cell cultures. Cell culture provides the required setting for the detection and identification of numerous pathogens of humans, which is achieved via virus isolation in the cell culture as the ?gold standard? for virus discovery. In this review, we summarized the views of researchers on the current role of cell culture technology in the diagnosis of human diseases. The technological advancement of recent years, starting with monoclonal antibody development to molecular techniques, provides an important approach for detecting presence of viral infection. They are also used as a baseline for establishing rapid tests for newly discovered pathogens. A combination of virus isolation in cell culture and molecular methods is still critical in identifying viruses that were previously unrecognized. Therefore, cell culture should be considered as a fundamental procedure in identifying suspected infectious viral agent.
机译：细胞培养涉及从自然环境中分离细胞（体内）以及随后在受控环境人工条件下（体外）生长的复杂过程。来自特定组织或器官的细胞作为短期或既定的细胞系进行培养，这些细胞系广泛用于研究和诊断，尤其是在病毒感染方面，因为病原性病毒分离取决于允许的细胞培养物的可用性。细胞培养为检测和鉴定人类多种病原体提供了所需的环境，这是通过在细胞培养中分离病毒作为“金标准”而实现的。用于发现病毒。在这篇综述中，我们总结了研究人员对细胞培养技术在人类疾病诊断中当前作用的观点。从单克隆抗体发展到分子技术开始，近年来的技术进步为检测病毒感染的存在提供了重要的方法。它们还用作建立新发现病原体快速测试的基准。细胞培养中的病毒分离与分子方法的结合对于识别以前未被识别的病毒仍然至关重要。因此，细胞培养应被视为鉴定可疑传染性病毒制剂的基本程序。

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