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IMMUNOLOGICAL ROLE OF BRUCELLA ABORTUS CELL WALLS

机译:布鲁氏菌细胞壁的免疫学作用

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Foster, John W. (University of Georgia, Athens) and Edgar Ribi. Immunological role of Brucella abortus cell walls. J. Bacteriol. >84:258–268. 1962—Cell walls and protoplasm were prepared from organisms disrupted in a refrigerated pressure cell. Cell walls were purified by sedimentation in a linear glycerol gradient. Antigens capable of protecting mice against infection with Brucella abortus and of reacting with antiserum prepared against whole cells were present chiefly in the cell wall; substances lethal to mice and responsible for primary inflammation of rabbit skin were also associated with the cell wall. Limited activity of protoplasm in these biological tests may or may not be due to contamination with cell-wall material. A substance extracted from whole cells with aqueous ether possessed an immunizing potency superior to that of killed whole cells or cell walls.
机译:Foster,John W.(雅典,乔治亚大学)和Edgar Ribi。流产布鲁氏菌细胞壁的免疫学作用。 J.细菌。 > 84: 258–268。 1962年-细胞壁和原生质是由在冷藏压力池中破裂的生物制备的。通过以线性甘油梯度沉淀来纯化细胞壁。能够保护小鼠免受流产布鲁氏菌感染的抗原并能与针对全细胞制备的抗血清反应的抗原主要存在于细胞壁中。对小鼠具有致命性并引起兔皮肤原发性炎症的物质也与细胞壁有关。在这些生物学测试中,原生质活动受限可能是由于细胞壁材料污染造成的,也可能不是。用含水乙醚从全细胞中提取的物质具有比杀死的全细胞或细胞壁更高的免疫力。

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