Foster, John W. (University of Georgia, Athens), Robert M. Cowan, and Ted A. Maag. Rupture of bacteria by explosive decompression. J. Bacteriol. >83:330–334. 1962.—A device is described for instantaneously rupturing bacteria and other cells in a closed system under controlled conditions by explosive decompression. With this device, 31 to 59% of Serratia marcescens, ranging up to 20 mg (dry wt) of cells per ml, were ruptured after nitrogen saturation at 1740 psi. Under similar conditions, 10 to 25% of Brucella abortus and Staphylococcus aureus were ruptured. Rupture of these organisms produced readily separable cell walls. Centrifugation in linear glycerol gradients was applied to further separate cell walls from debris. Mycoplasma gallinarum, Leptospira pomona, and Eimeria tenella (avian coccidia) o?cysts were also broken up by the decompression chamber. Pressure and duration of saturation of cells with gas affected rupture efficiency. Within the limits of this study, concentration of organisms and volume of suspensions did not have a definite effect.
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机译：Foster，John W.（雅典，乔治亚大学），Robert M. Cowan和Ted A. Maag。爆炸性减压使细菌破裂。 J.细菌。 > 83： 330-334。 1962年。描述了一种通过爆炸性减压在受控条件下瞬时破裂细菌和其他细胞的装置。使用此设备，在1740 psi的氮气饱和度下，破裂的31％至59％的粘质沙雷氏菌（Serratia marcescens）达20 mg（干重）每毫升细胞。在相似的条件下，流产的细小芽孢杆菌和金黄色葡萄球菌的10％至25％破裂。这些生物的破裂产生了易于分离的细胞壁。应用线性甘油梯度离心以进一步将细胞壁与碎片分离。减压室还将鸡支原体，波状钩端螺旋体和 Eimeria tenella （禽球菌）卵囊破裂。受气体影响的破裂效率的细胞压力和饱和持续时间。在这项研究的范围内，微生物的浓度和悬浮液的体积没有确定的影响。
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