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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Evaluation of Tumour Associated Macrophages and Angiogenesis in Ameloblastoma ZC33-ZC35
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Evaluation of Tumour Associated Macrophages and Angiogenesis in Ameloblastoma ZC33-ZC35

机译：评价成釉细胞瘤ZC33-ZC35的肿瘤相关巨噬细胞和血管生成。

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Introduction: Ameloblastoma is a locally invasive odontogenic neoplasm that has a high recurrence rate. The invasion of adjacent tissue is supported by angiogenesis stimulated by Tumour-Associated Macrophages (TAMs). TAMs are macrophages modified in the milieu of the tumour microenvironment and have very weak or no ability to present antigens. Thus, there is collaboration between the tumour and the tumour microenvironment to maintain tumour enlargement. TAMs exist as the classically activated M1 macrophages that possess antitumour activity and the otherwise activated M2 macrophages that support tumour invasion and metastasis. Aim: To investigate the relative expression and topography of TAMs and CD34 in ameloblastoma in order to assess their affiliation and effect on tumour growth.Materials and Methods: Forty-six Formalin Fixed Paraffin Embedded (FFPE) blocks of ameloblastoma were processed for Abcam Mouse monoclonal Anti-CCR7 antibody, Abcam Rabbit polyclonal Anti-CD206 antibody and Dako Mouse monoclonal Anti-CD34 antibody QBEnd-10. Cytoplasmic/membrane brown staining was taken as positive for all antibodies. The relative percentage of TAMs was classified as: 50%. TAMs related Microvessel Density (MVD) was evaluated as the mean of the three-recorded values. Cases with no CD34+ vessels adjacent to the TAMs region had MVD score of 0. Simple descriptive statistics was applied.Results: Macrophages adjacent to peri-tumour islands were marked by CD206 and CCR7 and we noted negligible intra-tumour presence of positive macrophages. The percentage of positive CCR7 immune cells was greater than that for CD206 in 38 (82.6%) cases, approximately equal to CD206 in 6 (13%) cases, and the CD206 expression was more than CCR7 in only 2 (4.3%) cases. In 34 (73.9%) cases, the area of MVD did not overlap with the region of TAMs but in 4 (8.7%) cases (where MVD overlapped TAM1), the average MVD score was 20.Conclusion: The relative percentage of TAM1 exceeds TAM2 in peri-tumoural areas of ameloblastoma, conferring anti-angiogenic and hence anti-tumour activity on the tumour.
机译：简介：成釉细胞瘤是一种局部浸润性牙源性肿瘤，具有较高的复发率。肿瘤相关巨噬细胞（TAM）刺激血管生成，支持相邻组织的浸润。 TAM是在肿瘤微环境的环境中修饰的巨噬细胞，并且具有非常弱的或没有呈递抗原的能力。因此，在肿瘤和肿瘤微环境之间存在协作以维持肿瘤扩大。 TAM以具有抗肿瘤活性的经典激活的M1巨噬细胞和支持肿瘤侵袭和转移的其他激活的M2巨噬细胞形式存在。目的：研究成纤维细胞瘤中TAMs和CD34的相对表达和形貌，以评估其与肿瘤生长的关系。材料与方法：对成纤维细胞瘤中46个福尔马林固定石蜡包埋（FFPE）块进行Abcam Mouse单克隆治疗抗CCR7抗体，Abcam Rabbit多克隆抗CD206抗体和Dako小鼠抗CD34单克隆抗体QBEnd-10。细胞质/膜棕色染色被视为所有抗体均为阳性。 TAM的相对百分比分类为：50％。 TAM相关的微血管密度（MVD）被评估为三个记录值的平均值。 TAMs区域附近无CD34 +血管的病例的MVD评分为0。采用简单的描述性统计方法。结果：肿瘤周围岛附近的巨噬细胞以CD206和CCR7标记，我们注意到肿瘤内阳性巨噬细胞的存在可忽略不计。在38（82.6％）例中，阳性CCR7免疫细胞百分比高于CD206，在6（13％）例中大约等于CD206，而CD206的表达仅在2（4.3％）例中高于CCR7。在34（73.9％）例中，MVD面积与TAM区域不重叠，但在4（8.7％）例中（MVD与TAM1重叠），MVD平均得分为20。结论：TAM1的相对百分比超过TAM2在成纤维细胞瘤的肿瘤周围区域中，赋予抗血管生成作用，因此对肿瘤具有抗肿瘤活性。

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