Purple nonsulfur photosynthetic bacteria were cultured anaerobically in the absence of light by a modification of the Hungate technique. Growth was slow and resembled that of fastidious anaerobes; on yeast extract-peptone-agar medium, each cell produced about 16 descendants in 15 to 20 days. Growth was stimulated by addition of ethyl alcohol, acetate and H2, or pyruvate and H2. Cells grown in the presence of pyruvate and H2 produced acetate and CO2; each cell produced approximately 10 descendants in 24 hr under anaerobic, dark conditions. Spectrophotometric evidence obtained from cells which were the product of five generations suggests no difference between the bacteriochlorophyll and carotenoids synthesized by cells grown anaerobically under dark or light conditions. Likewise, the ultrastructure of the photosynthetic apparatus in cells grown anaerobically in the dark and in the light appears similar.
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