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首页> 外文期刊>Journal of bacteriology >Effects of Supersuppressor Genes on Enzymes Controlling Lysine Biosynthesis in Saccharomyces
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Effects of Supersuppressor Genes on Enzymes Controlling Lysine Biosynthesis in Saccharomyces

机译：超抑制基因对酿酒酵母控制赖氨酸合成的酶的影响

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Yeast supersuppressor genes capable of masking the effects of several lysine mutant genes (ly1-1, ly9-1, ly2-1) were studied with respect to their effects on the respective enzymes (saccharopine dehydrogenase, saccharopine reductase, and α-amino-adipic acid reductase). In all strains tested, the supersuppressors functioned by allowing enzyme synthesis not found in the unsuppressed mutant. Studies by optical methods of saccharopine dehydrogenase and saccharopine reductase extracted from suppressed ly1-1 and ly9-1 cells, respectively, revealed that the Km values for these enzymes were significantly greater than those found in wild type. Saccharopine dehydrogenase from suppressed ly9-1 cells was found to have Km values similar to wild type. These findings are consistent with the inference that a supersuppressor may act by enabling nonsense codons to be read, producing altered enzyme protein. Recent findings that lysine degradation in mammals may involve saccharopine and that the human diseases, hyperlysinemia and saccharopinuria, may be due to metabolic blocks in this route of lysine degradation suggest the ly1-1 and ly9-1 yeast mutants as models for the human condition and its possible euphenic treatment.
机译：酵母超抑制基因能够掩盖几种赖氨酸突变基因（ ly 1-1 ， ly 9-1 ， ly 2-1 ）对它们对相应酶（糖精脱氢酶，糖精还原酶和α-氨基己二酸还原酶）的影响进行了研究。在所有测试的菌株中，超级抑制剂通过允许未抑制突变体中未发现的酶合成而起作用。光学方法从抑制的 ly 1-1 和 ly 9-1 提取的糖精脱氢酶和糖精还原酶的研究细胞分别显示这些酶的 K m 值显着高于野生型。发现抑制的 ly 9-1 细胞中的糖精脱氢酶具有与野生型相似的 K m 值。这些发现与推论者可能通过使无义密码子被阅读，产生改变的酶蛋白而起作用的推论一致。最近的发现表明，赖氨酸在哺乳动物中的降解可能与糖醋栗有关，而人类疾病，高溶血血症和糖尿症可能是由于赖氨酸降解途径中的代谢受阻所致，这表明 ly 1-1 和 ly 9-1 酵母突变体可作为人类疾病及其可能的中性治疗的模型。

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