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首页> 外文期刊>Journal of bacteriology >Ribonucleic Acid Synthesis During Microcyst Formation in Myxococcus xanthus: Characterization by Deoxyribonucleic Acid-Ribonucleic Acid Hybridization
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Ribonucleic Acid Synthesis During Microcyst Formation in Myxococcus xanthus: Characterization by Deoxyribonucleic Acid-Ribonucleic Acid Hybridization

机译:粘液球菌微囊形成过程中核糖核酸的合成:脱氧核糖核酸-核糖核酸杂交的表征。

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摘要

The technique of deoxyribonucleic acid-ribonucleic acid (RNA) hybridization was used to compare the RNA synthesized during vegetative growth and microcyst formation in Myxococcus xanthus. All classes of RNA, including ribosomal RNA, were synthesized during microcyst formation. The results indicate that the ribosomal RNA synthesized during microcyst formation was indistinguishable from that made during vegetative growth. Hybridization competition experiments demonstrated that certain messenger RNA species are synthesized only during vegetative growth, whereas others are synthesized only during microcyst formation. The synthesis of a new species of RNA polymerase does not appear to be responsible for differential transcription during morphogenesis in M. xanthus since the rifampicin sensitivity of transcription was conserved during microcyst formation.
机译:利用脱氧核糖核酸-核糖核酸(RNA)杂交技术,比较了粘球菌在营养生长和微囊形成过程中合成的RNA。微囊形成过程中合成了所有类别的RNA,包括核糖体RNA。结果表明,在微囊形成过程中合成的核糖体RNA与在营养生长过程中合成的核糖体RNA是没有区别的。杂交竞争实验表明,某些信使RNA仅在营养生长期间合成,而其他信使RNA仅在微囊形成期间合成。 RNA聚合酶的新物种的合成似乎不负责在 M的形态发生过程中的差异转录。黄腐质,因为在微囊形成过程中,利福平的转录敏感性得以保留。

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