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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Phenotypic and Molecular Characterization of Plasmid Mediated AmpC among Clinical Isolates of Klebsiella pneumoniae Isolated from Different Hospitals in Tehran
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Phenotypic and Molecular Characterization of Plasmid Mediated AmpC among Clinical Isolates of Klebsiella pneumoniae Isolated from Different Hospitals in Tehran

机译：德黑兰不同医院分离的肺炎克雷伯菌临床分离株中质粒介导的AmpC的表型和分子表征

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Introduction: Klebsiella pneumoniae is one of the main opportunistic pathogens which can cause different types of infections. Production of beta-lactamases like AmpC and ESBL mostly lead to beta-lactam resistance in these Gram-Negative bacteria. The aim of this study was the detection of AmpC-producing K. pneumoniae in clinical isolates. Materials and Methods: Three hundred and three isolates of K. pneumoniae were identified. Double disc method including cefoxitin with cefepime and using boronic acid with cloxacillin were performed as two phenotypic methods for detection of AmpC. Amplification of AmpC gene was performed by PCR. Results: Eight and three isolates showed positive results in double disc method and by using boronic acid with cloxacillin, respectively. Five isolates had specific band for AmpC gene after electrophoresis. Conclusion: Our results were indicated the low prevalence of AmpC-producer-K. pnemoniae in Iran. On the other hand these two tested phenotypic methods showed low sensitivity for detection of AmpC.
机译：简介：肺炎克雷伯菌是主要的机会性病原体之一，可以引起不同类型的感染。在这些革兰氏阴性细菌中，β-内酰胺酶（如AmpC和ESBL）的产生主要导致β-内酰胺抗性。这项研究的目的是在临床分离株中检测产生AmpC的肺炎克雷伯菌。材料与方法：鉴定出303株肺炎克雷伯菌。作为检测AmpC的两种表型方法，采用了头孢西丁与头孢吡肟，硼酸与氯西林联用的双盘法。通过PCR进行AmpC基因的扩增。结果：分别采用双盘法和硼酸与氯西林联用的八株和三株分离株均显示阳性结果。电泳后有5株分离株具有针对AmpC基因的特异性条带。结论：我们的结果表明AmpC-producer-K的患病率较低。伊朗的妖精。另一方面，这两种测试的表型方法对AmpC的检测灵敏度较低。

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