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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Can Feulgen Stain be a Reliable Biomarker over PAP Stain for Estimation of Micronuclei Score?
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Can Feulgen Stain be a Reliable Biomarker over PAP Stain for Estimation of Micronuclei Score?

机译：Feulgen染色可以比PAP染色可靠的生物标志物，以评估微核得分吗？

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Introduction: Malignant transformation of the Potentially Malignant Lesions (PML) in the oral cavity is associated with elevated mortality rate because of its aggressive and exceedingly invasive nature. Meticulous diagnosis and prompt therapy of PML may help prevent malignant conversion in oral lesions. Carcinogenic insult to oral cells results in chromosomal damage and formation of Micronuclei (Mn), before the development of clinical symptoms. Aim: To determine the genotoxic effect of smoking and chewing tobacco on target tissue using Mn assay and to evaluate the prevalence of other nuclear anomalies associated with it and to determine the reliability of feulgen stain for Mn assay over Papaincolau (PAP) stain. Materials and Methods: PAP and feulgen staining was done to study Mn in individuals who were having tobacco habits (smoking and chewing) without lesion (n=30), individuals who were having tobacco habit (smoking and chewing) with PML (n=30) and apparently healthy subjects (n=30). Data was analysed for statistical significance using SPSS 17.0 by Kruskal - Wallis Test and Bonferronii test. Results: Tobacco habits in the form of smoking and chewing have mutagenic effects on human chromosomes which is indicated by increased frequency of Mn in oral exfoliative cells. The mean Mn frequency using feulgen stain was found to be 12.27 with lesion, 10.23 with without lesion and 3.87 in controls. Whereas, metanucleated analysis revealed no significant correlation with the formation of Mn. Non-specific DNA stain (PAP) showed high numbers of Mn cells in all the groups compared to feulgen. Statistically significant difference (p<0.0001) was observed when both the stains were compared for Mn numbers. Conclusion: These findings indicate that the individuals having tobacco habits (smoking and chewing) with lesion have high number of Mn cells, thus supporting the assay to be used as a reliable biomarker to assess the genotoxic effect of tobacco in the oral mucosa. The reason for almost twice as high Mn in PAP stained smears is suggestive of cell injury which is collimated by formation of keratin bodies, resulting in its misinterpretation as Mn, leading to false positive results. Hence, it was concluded that PAP stain can be used to identify abnormal cytological changes resulting from mutagenic agent but not to interpret Mn.
机译：简介：口腔中潜在恶性病变（PML）的恶性转化与侵袭性和过度侵袭性有关，因此死亡率升高。 PML的细致诊断和及时治疗可能有助于防止口腔病变恶变。在临床症状出现之前，对口腔细胞的致癌侮辱会导致染色体损伤和微核（Mn）形成。目的：使用锰测定法确定吸烟和咀嚼烟草对目标组织的遗传毒性作用，并评估与之相关的其他核异常的发生率，并确定用于锰测定法的富尔根染料优于帕帕因科劳（PAP）染料的可靠性。材料和方法：进行PAP和feulgen染色以研究具有吸烟习惯（吸烟和咀嚼）但无病变的个体（n = 30），具有吸烟习惯（吸烟和咀嚼）的PML个体（n = 30）中的Mn。 ）和显然健康的受试者（n = 30）。通过Kruskal-Wallis检验和Bonferronii检验，使用SPSS 17.0分析数据的统计显着性。结果：吸烟和咀嚼形式的烟草习性对人类染色体具有诱变作用，这可通过口腔脱落细胞中Mn频率增加来表明。发现使用富尔根染料的平均Mn频率为有病变的12.27，无病变的10.23和对照组的3.87。然而，有核分析显示与锰的形成没有显着相关性。与feulgen相比，非特异性DNA染色（PAP）在所有组中均显示出大量的Mn细胞。当将两种染色剂的Mn值进行比较时，观察到统计学上的显着差异（p <0.0001）。结论：这些发现表明，具有吸烟习惯（吸烟和咀嚼）并有病变的个体具有大量的Mn细胞，从而支持该测定作为评估口腔黏膜中烟草遗传毒性作用的可靠生物标记物。 PAP染色涂片中Mn含量几乎高出两倍的原因表明，细胞损伤通过形成角蛋白小体而得以准直，导致其误解为Mn，从而导致假阳性结果。因此，得出的结论是，PAP染色可用于鉴定由诱变剂引起的异常细胞学变化，但不能用于解释Mn。

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