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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Cymbopogon Citratus (Lemongrass Oil) Oral Sprays as Inhibitors of Mutans Streptococci Biofilm Formation
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Cymbopogon Citratus (Lemongrass Oil) Oral Sprays as Inhibitors of Mutans Streptococci Biofilm Formation

机译：柠檬草油口服液作为突变体链球菌生物膜形成的抑制剂

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Dental caries that occurs in young children or Early Childhood Caries (ECC) remains highly prevalent worldwide. Streptococcus mutans is not only one of the pioneer groups in plaque formation but is also crucial for its continuous development. It is important to prevent the presence of Mutans Streptococci (MS) for more effective prevention of dental caries.Aim: To evaluate the susceptibility of Streptococcus mutans (ATCC 25175), Streptococcus sobrinus (ATCC 6715) and three Streptococcus mutans clinical isolates from Thai children to three oral spray formulations (2%L38, 4%L40, 6%L42) of Cymbopogon citratus extracted oil, and evaluate the inhibitory effect of each formulation on growth and biofilm formation of each strain.Materials and Methods: S. mutans clinical isolations were obtained from children (12 males, 5 females aged 3.5-10-years-old) who came to the Paediatric Dental Clinic, Faculty of Dentistry, Chulalongkorn University, Bangkok. The inhibitory effect of each formulation on growth and biofilm formation was performed using a 96-well polystyrene plate biofilm assay. The inhibition effect was further analysed using fluorescent dyes (SYTO9 and propidium iodide) and Confocal Laser Scanning Microscopy (CLSM). All experiments were performed in triplicate. Data was expressed as mean±SD. For the determination of live/dead cells at the excitation wavelength of 485 nm, the green emission at 530 nm (live cells) and the red emission at 630 nm (dead cells) were measured and their ratio was calculated. The Kruskal-Wallis Test (Bonferroni and Dunn Test) was used to compare the experimental and control groups (significance at 95%).Results: All oral spray formulations showed excellent antibacterial activity. Results showed that 3 and 6 hours. preformed biofilms of all strains were sensitive to all formulations (100%) when compared to the controls. Biofilm formation of all strains was inhibited (100%) when compared to the controls by all formulations at different concentrations. Six percent (L42) oil formulations showed the maximum inhibition effect, followed by 4% (L40) and 2% (L38), respectively. Results from CLSM analysis of S. mutans (ATCC 25175) showed that 6% (L42) oil formulations gave the maximum inhibition effect, followed by 4% (L40) and 2% (L38), respectively. For S. sobrinus (ATCC 6715), 2% (L38) oil formulations showed the maximum inhibition effect, followed by 4% (L40) and 6% (L42). For all S. mutans clinical isolates, all formulations inhibited biofilm effectively and were not significantly different.Conclusion: In this study, all oral spray formulations from Cymbopogon citratus essential oil showed excellent antibacterial activity and they might be an alternative inexpensive natural medication to prevent dental caries in the near future.
机译：在幼儿或幼儿龋齿（ECC）中发生的龋齿在世界范围内仍然很普遍。变形链球菌不仅是噬菌斑形成的先驱者之一，而且对于其持续发展也至关重要。重要的是要防止变形链球菌（MS）的存在，以更有效地预防龋齿。目的：评估变形链球菌（ATCC 25175），链球菌（ATCC 6715）和三种变形链球菌临床分离株的敏感性。从泰国儿童到3种口服柠檬角柏提取物的口服喷雾制剂（2％L38、4％L40、6％L42），并评估每种制剂对每种菌株的生长和生物膜形成的抑制作用。材料和方法：变形链球菌临床分离物来自于曼谷朱拉隆功大学牙科系儿科牙科诊所的儿童（12名男性，5名女性，年龄在3.5-10-岁之间）。使用96孔聚苯乙烯平板生物膜测定法执行每种制剂对生长和生物膜形成的抑制作用。使用荧光染料（SYTO9和碘化丙啶）和共聚焦激光扫描显微镜（CLSM）进一步分析了抑制作用。所有实验均重复三次。数据表示为平均值±SD。为了确定激发波长为485 nm的活/死细胞，测量了530 nm的绿色发射（活细胞）和630 nm的红色发射（死细胞），并计算了它们的比率。使用Kruskal-Wallis试验（Bonferroni和Dunn试验）比较实验组和对照组（在95％时具有显着性）。结果：所有口服喷雾制剂均显示出优异的抗菌活性。结果显示分别为3和6小时。与对照相比，所有菌株的预先形成的生物膜对所有制剂均敏感（100％）。当与不同浓度的所有制剂相比，与对照相比，所有菌株的生物膜形成均被抑制（100％）。 6％（L42）油配方显示出最大的抑制作用，其次分别为4％（L40）和2％（L38）。 CL。变形链球菌（ATCC 25175）的分析结果表明，6％（L42）油配方具有最大的抑制作用，其次分别是4％（L40）和2％（L38）。对于so。sobrinus（ATCC 6715），2％（L38）油配方显示出最大的抑制作用，其次是4％（L40）和6％（L42）。对于所有变形链球菌临床分离株，所有制剂均能有效抑制生物膜，且无显着差异。以防止在不久的将来出现龋齿。

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