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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study
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Expression of CCK Receptors in Carcinoma Gallbladder and Cholelithiasis: A Pilot Study

机译：CCK受体在胆囊癌和胆石症中的表达：一项初步研究。

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Background: Gastrin and cholecystokinin (CCK) receptors are trophic for various gastrointestinal malignancies. Their role in gallbladder cancer has not been widely studied. Objectives: To identify expression of CCK-A and CCK-B receptors in the tissue and blood of patients suffering from carcinoma (CA) gallbladder and gallstone disease and to compare expression of CCK A and B receptors in the gall bladder tissue and blood of healthy individuals and patients of CA gallbladder, and gallstone diseases. Materials and Methods: Forty nine subjects of both genders were recruited, comprising of 22 patients of CA gall bladder, 19 cases of cholelithiasis and, 8 normal gallbladders obtained from patients operated for trauma of the biliary system or Whipple?s procedure. RNA extraction and cDNA formation for CCK-A and CCK-B receptors were carried out. Real Time PCR was performed on cDNA and threshold cycle (Ct) value of each sample was obtained and ?Ct was calculated. Chi-square test for comparing two groups and ANOVA test for comparing multiple groups were applied and if p0.05). Conclusion: This preliminary study showed higher expression of CCK-A receptors in patients of cholelithiasis and decreased expression of CCK-A receptors in patients of CA gallbladder as compared to normal gallbladder although it did not rise to statistical significance.
机译：背景：胃泌素和胆囊收缩素（CCK）受体对于各种胃肠道恶性肿瘤都是营养性的。它们在胆囊癌中的作用尚未得到广泛研究。目的：鉴定胆囊癌和胆结石病患者的组织和血液中CCK-A和CCK-B受体的表达，比较健康人胆囊组织和血液中CCK-A和B受体的表达。胆囊癌和胆结石疾病的个人和患者。材料和方法：招募了49位男女受试者，包括22例CA胆囊癌，19例胆石症和8例从胆道系统创伤或Whipple's手术患者获得的正常胆囊。对CCK-A和CCK-B受体进行RNA提取和cDNA形成。对cDNA进行实时PCR，获得每个样品的阈值循环（Ct）值并计算ΔCt。卡方检验用于比较两组，ANOVA检验用于比较多个组（如果p0.05）。结论：这项初步研究显示，胆囊结石症患者的CCK-A受体表达高于正常胆囊，而胆囊结石患者的CCK-A受体表达却降低，尽管这没有统计学意义。

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