...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Prevalence of Specific Helicobacter Pylori cagA, vacA, iceA, ureC Genotypes and its Clinical Relevance in the Patients with Acid-Peptic Diseases DC23-DC26
【24h】

Prevalence of Specific Helicobacter Pylori cagA, vacA, iceA, ureC Genotypes and its Clinical Relevance in the Patients with Acid-Peptic Diseases DC23-DC26

机译：酸性消化系统疾病患者中特定幽门螺杆菌cagA，vacA，iceA，ureC基因型的流行及其临床相关性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: Virulent markers of H. pylori, the vacuolating cytotoxin (vacA), cytotoxin-associated gene A (cagA), induced by contact with epithelium factor antigen (iceA gene) and the urease C gene (ureC) may plays a major role in determining the clinical outcome of Helicobacter infections.Aim: To detect the prevalence of the cagA, vacA, ureC and iceA genotypes of H. pylori from antral biopsy specimens of patients and to associate its role in specific disease.Materials and Methods: The study was conducted at Department of Microbiology of Shree P.M. Patel College of Paramedical Sciences, Anand, Gujarat, India. Seventy one antral biopsies of symptomatic patients referred for endoscopy from October 2012 to September 2013 were subjected to Multiplex PCR. DNA isolation from 71 biopsy samples was done by using ?QIAamp DNA mini kit? from QIAGEN (GmbH, Hilden, Germany). Data was analysed using Chi square (?2) test and p-value<0.05 was considered significant.Results: Out of the 71 biopsies screened, 22(31%) samples were positive for H. pylori by PCR, with high proportion of cagA positive (17/22 specimen; 77.27%), followed by ureC positive (4/22 specimen; 18.18%) and vacA positive (1/22 specimen; 4.54%) strains. Significant association was found between cagA and female gender (p-value=0.042). Out of 17 cagA positive strains, 9(52.94%) were found in patients with gastritis, 5(29.41%) in reflux oesophagitis and 3(17.64%) in patients with diodenal ulcer. We found 0% prevalence of iceA gene; conversely we had three peptic ulcer patients with only cagA positivity.Conclusion: The cagA positive strain mainly affects the patients with gastritis specifically of female gender and iceA genotype is not a useful marker associated with peptic ulcer disease. Patients should be screened for cagA genotype when reported to be a case of gastritis for early treatment to prevent further complications such as cancer.
机译：简介：幽门螺杆菌，空泡细胞毒素（vacA），细胞毒素相关基因A（cagA），与上皮因子抗原（iceA基因）和脲酶C基因（ureC）接触所致的强力标志物可能在以下方面起主要作用目的：从患者的活检样本中检测幽门螺杆菌cagA，vacA，ureC和iceA基因型的流行情况，并确定其在特定疾病中的作用。在Shree PM微生物学系进行印度古吉拉特邦阿南德，帕特尔辅助医学学院。对2012年10月至2013年9月接受内窥镜检查的有症状患者的71例肛门活检进行了多重PCR。使用“ QIAamp DNA mini kit”从71个活检样品中分离出DNA。由QIAGEN（GmbH，Hilden，Germany）提供。使用卡方检验（？2）分析数据，p值<0.05被认为是显着的。结果：在71份活检样本中，有22份（31％）样本的幽门螺杆菌呈阳性，其中cagA含量较高阳性（17/22标本; 77.27％），其次是ureC阳性（4/22标本; 18.18％）和vacA阳性（1/22标本; 4.54％）。发现cagA与女性之间存在显着关联（p值= 0.042）。在17株cagA阳性菌株中，胃炎患者发现9株（52.94％），反流性食管炎患者发现5株（29.41％），十二指肠溃疡患者发现3株（17.64％）。我们发现iceA基因的患病率为0％;相反，我们有3例消化性溃疡患者只有cagA阳性。结论：cagA阳性菌株主要影响女性的胃炎，尤其是女性，而iceA基因型不是与消化性溃疡疾病相关的有用标志物。当报告为早期治疗的胃炎病例时，应筛查cagA基因型，以防止进一步的并发症，例如癌症。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号