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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Correlation of Plasma NeutrophilElastase Activity and EndogenousProtease Inhibitor Levels with theSeverity of Pre-eclampsia BC09-BC12
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Correlation of Plasma NeutrophilElastase Activity and EndogenousProtease Inhibitor Levels with theSeverity of Pre-eclampsia BC09-BC12

机译：子痫前期的严重程度与血浆中性粒细胞弹性蛋白酶活性和内源性蛋白酶抑制剂水平的相关性

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Introduction: Pre-eclampsia (PE) is a common maternal syndrome characterized by severe systemic inflammatory response including neutrophil activation leading to uncontrolled activity of elastase. The excessive activity of elastase would lead to destroyal of the integrity of endothelial cells and could exacerbate the pathophysiological symptoms in PE. Thus, assessment of NE activity and its control mechanisms would be of relevance in the determination of severity of PE. Aim: To correlate the activity of plasma NE and its endogenous inhibitors a1-antitrypsin (a1-AT) and a2-macroglobulin (a2-MG) with severity of PE. Materials and Methods: A comparative study was conducted between normotensive pregnant (n=50) and pre-eclamptic (n=50) women. Serum C-Reactive Protein (CRP) was estimated by rapid latex slide and uric acid by uricase method. Plasma elastase was estimated using succinyl tri- L-alanyl-p-nitroanilide as substrate. Plasma a1-AT, a2-MG and NE- a1-AT complex were quantified by ELISA. ANOVA and Pearson?s correlation tests were used to analyze the data. The results were expressed as mean±SD and p-value <0.001 was considered statistically highly significant. Results: The activity of elastase was increased significantly in severe PE (0.62±0.08) in comparison to normal (0.35±0.10) and mild pre-eclamptic subjects (0.37±0.03). The values of a1-AT were significantly less in mild (83.94±25.08) and severe PE (68.58+26.39) in comparison to normal (110.26±42.39). There was a significant rise in the levels of a2-MG in severe PE. However, the complex estimation did not evince any significant changes. Conclusion: The results of the present study indicate a significantly elevated elastase activity, a2-MG levels and decreased a1-AT in severe PE patients. The correlation analyses of PE severity parameters with NE, a1-AT and a2-MG further support the roles of these molecules in the assessment of severity of PE.
机译：简介：子痫前期（PE）是一种常见的产妇综合症，其特征是严重的全身性炎症反应，包括嗜中性粒细胞活化，导致弹性蛋白酶的活性不受控制。弹性蛋白酶的过度活性将导致内皮细胞完整性的破坏，并可能加剧PE的病理生理症状。因此，对NE活动及其控制机制的评估与确定PE严重程度有关。目的：将血浆NE及其内源性抑制剂α1-抗胰蛋白酶（α1-AT）和α2-巨球蛋白（α2-MG）的活性与PE的严重程度相关联。材料和方法：在正常血压孕妇（n = 50）和先兆子痫（n = 50）妇女之间进行了比较研究。血清C反应蛋白（CRP）是通过快速乳胶片和尿酸通过尿酸酶法估算的。使用琥珀酰三L-丙氨酰基-对硝基苯胺作为底物估计血浆弹性蛋白酶。通过ELISA定量血浆a1-AT，a2-MG和NE-a1-AT复合物。方差分析和皮尔逊相关测试用于分析数据。结果表示为平均值±SD，p值<0.001被认为在统计学上高度显着。结果：与正常人（0.35±0.10）和轻度先兆子痫患者（0.37±0.03）相比，重度PE（0.62±0.08）的弹性蛋白酶活性显着增加。与正常人（110.26±42.39）相比，轻度（83.94±25.08）和重度PE（68.58 + 26.39）的a1-AT值显着降低。重度PE患者的a2-MG水平显着升高。但是，复杂的估计没有明显的变化。结论：本研究结果表明，重度PE患者的弹性蛋白酶活性，a2-MG水平显着升高和a1-AT降低。 PE严重性参数与NE，a1-AT和a2-MG的相关性分析进一步支持了这些分子在PE严重性评估中的作用。

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