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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Significance of Tumour Budding with Cytokeratin 20 Immunostaining as a Histopathological Prognostic Marker in Colorectal Adenocarcinoma
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Significance of Tumour Budding with Cytokeratin 20 Immunostaining as a Histopathological Prognostic Marker in Colorectal Adenocarcinoma

机译：细胞角蛋白20免疫染色作为组织病理学预后标志物在结肠直肠腺癌中的意义。

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Tumour Budding (TB) in Colorectal Carcinoma (CRC) has been studied comprehensively and brought TB into the clinical dominion as an additional prognostic parameter. The tumour aggressiveness with poorer survival of CRC patient is linked to presence of Peritumoural Budding (PTB) at the invasion front and Intratumoural Budding (ITB) in the tumour centre.Aim: To study and compare the scoring system of TB by Haematoxylin and Eosin (H&E) staining with immunohistochemistry (IHC) CK20 in resected specimens of CRC and, its relation between TB grades with known prognostic histopathological parameters of CRC.Materials and Methods: The descriptive cross-sectional study was conducted in JSS Medical College and Hospital, Mysuru, Karnataka, India. A total of 50 cases of CRC were studied prospectively over a period of two years. Both H&E and IHC CK20 stained slides were studied. A total of 10 high power field (10 HPF) in invasive front were evaluated for PTB10HPF and within the tumour for ITB10HPF. The high dense area which had the maximum TB was taken as Hot Spot (HS). Continuous and cut off values of PTB, ITB and OTB (overall tumour budding) scores were compared in both H&E and IHC stained sections and also evaluated the association with various clinicopathological prognostic features.Results: An increase in the number of TB (PTB-46%, ITB-72%, OTB-28%), via IHC was noted. On continuous scores PTB, ITB and OTB counts showed significant association with tumour stage, lymph node metastasis and lymphovascular invasion (all p<0.05). When cut-offs were applied these statistical significance were lost. The Pearson?s correlation coefficient confirmed that OTB is better in evaluating TB than PTB and ITB.Conclusion: TB is a potential and potent histopathological prognostic forecaster of lymph nodal metastasis and a higher stage of tumour and allocates as a marker for stratification of patients into high risk categories in CRC. The TB in routine histopathology report should be advocated as H&E staining aided by IHC show significant association with histopathological parameters.
机译：已对结直肠癌（CRC）中的肿瘤发芽（TB）进行了全面研究，并将其作为临床的附加预后参数纳入了临床。 CRC患者的生存能力较差，其侵袭性与侵袭前的周围膜芽生（PTB）和肿瘤中心的瘤内芽生（ITB）有关。目的：研究和比较苏木精对结核的评分系统免疫组织化学（IHC）CK20和Eosin（H＆E）染色在CRC切除标本中的发现及其与TB等级与CRC的预后组织病理学参数之间的关系。材料与方法：描述性横断面研究是在JSS Medical上进行的印度卡纳塔克邦米苏鲁大学医院。在过去的两年中，共对50例CRC进行了研究。研究了H＆E和IHC CK20染色的玻片。评估了侵入性前线中总共10个高功率场（10 HPF）的PTB10HPF和肿瘤内的ITB10HPF。将具有最大TB的高密度区域作为热点（HS）。在H＆E和IHC染色切片中比较了PTB，ITB和OTB（总体肿瘤出芽）评分的连续和截止值，并评估了其与各种临床病理预后特征的相关性。结果：TB数量增加（通过IHC注意到PTB-46％，ITB-72％，OTB-28％）。在连续评分中，PTB，ITB和OTB计数与肿瘤分期，淋巴结转移和淋巴管浸润显着相关（所有p <0.05）。当采用临界值时，这些统计意义就会丢失。皮尔逊相关系数证实，OTB在评估结核病方面比PTB和ITB更好。结论：结核病是淋巴结转移和肿瘤更高分期的潜在且有效的组织病理学预后预测指标，可作为分层的标志物在CRC中属于高风险类别的患者应提倡在常规组织病理学报告中使用TB，因为IHC辅助的H＆E染色显示与组织病理学参数显着相关。

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