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首页> 外文期刊>Journal of bacteriology >DEGRADATION OF DEOXYRIBONUCLEIC ACID AND ALTERATION OF NUCLEIC ACID METABOLISM IN SUSPENSION CULTURES OF L-M CELLS INFECTED WITH EQUINE ABORTION VIRUS
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DEGRADATION OF DEOXYRIBONUCLEIC ACID AND ALTERATION OF NUCLEIC ACID METABOLISM IN SUSPENSION CULTURES OF L-M CELLS INFECTED WITH EQUINE ABORTION VIRUS

机译：感染流产病毒的L-M细胞悬浮培养中脱氧核糖核酸的降解和核酸代谢的改变。

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Randall, Charles C. (University of Mississippi School of Medicine, Jackson) and Barbara M. Walker. Degradation of deoxyribonucleic acid and alteration of nucleic acid metabolism in suspension cultures of L-M cells infected with equine abortion virus. J. Bacteriol. >86:138–146. 1963.—Metabolic alterations in log-phase suspension cultures infected with equine abortion virus (EAV) were determined in L-M cells simultaneously labeled or prelabeled with H3- or C14-thymidine. Although infection produced an early stimulation of the uptake of labeled thymidine (TdR) into the acid-soluble fraction of concurrently labeled cells, incorporation of the isotope into deoxyribonucleic acid (DNA) was progressively inhibited. The specific activity of infected-cell DNA was 48% of the control at 24 hr. The rate of incorporation of isotope from 12 to 24 hr was 43 and 13 counts per min per μg of DNA per hr for control and infected cultures, respectively. Owing to degradation of DNA, synthesis could not be accurately determined with the concurrently labeled cells. On the other hand, with prelabeled cells, quantitative isotopic methods could be used to determine the amount of DNA synthesized by measuring dilution of specific activity, even though infection triggered degradation of DNA into acid-soluble components. With this method, the DNA synthesized in infected cultures for 24 hr was approximately five times greater than the slight net increase determined by the diphenylamine reaction. The specific activity of infected-cell DNA decreased and then remained fixed after 24 hr, with 53% of the radioactivity appearing in the medium by 48 hr. No radioactive CO2 was detected as a consequence of DNA degradation. Infected cells lost ribonucleic acid (RNA) as well as DNA; RNA and DNA were reduced by 64 and 50%, respectively, at 48 hr. The degradation of DNA was effectively inhibited by chelating agents in situ and is thought to be due to a deoxyribonuclease. Preliminary experiments with extracts of infected cells support this observation. The relationship of deoxyribonuclease to the synthesis of viral DNA remains to be determined.
机译：Randall，Charles C.（密西西比大学医学院，杰克逊）和Barbara M. Walker。马堕胎病毒感染的L-M细胞悬浮培养物中脱氧核糖核酸的降解和核酸代谢的改变。 J.细菌。 > 86： 138-146。 1963年。在同时标记或预先标记有H 3 -或C 14 -的LM细胞中，确定了感染对马期流产病毒（EAV）的对数期悬浮培养物中的代谢变化。胸苷。尽管感染可以早期刺激被标记的胸腺嘧啶核苷（TdR）吸收到同时标记的细胞的酸溶级分中，但是同位素逐渐被抑制进入脱氧核糖核酸（DNA）中。在24小时时，感染细胞DNA的比活性是对照的48％。对照和感染培养物中，同位素掺入的速率为每分钟每微克DNA每小时12至24小时的计数分别为43和13个计数。由于DNA的降解，无法用同时标记的细胞准确地确定合成。另一方面，对于预先标记的细胞，即使感染导致DNA降解成酸溶性成分，也可以使用定量同位素方法通过测量比活度稀释来确定合成的DNA量。用这种方法，在感染的培养物中合成24小时的DNA大约是二苯胺反应确定的轻微净增加的五倍。受感染细胞DNA的比活度降低，然后在24小时后保持固定，到48小时，在培养基中出现53％的放射性。 DNA降解未检测到放射性CO 2 。被感染的细胞失去了核糖核酸（RNA）和DNA。在48小时时，RNA和DNA分别减少了64％和50％。螯合剂原位有效抑制了DNA的降解，这被认为是由于脱氧核糖核酸酶所致。感染细胞提取物的初步实验支持这一观察。脱氧核糖核酸酶与病毒DNA合成的关系尚待确定。

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