Mode of Action of Pectic Enzymes II. Further Purification of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans

L. Miller; J. D. Macmillan

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Mode of Action of Pectic Enzymes II. Further Purification of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans

机译：果胶酶的作用方式II。从多发酵梭菌中进一步纯化外半乳糖醛酸裂合酶和果胶酯酶

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Exopolygalacturonate lyase and pectinesterase from Clostridium multifermentans were purified 156-fold and 178-fold, respectively, by gel filtration chromatography on Sephadex G-200. The activities of both enzymes coincided in a single protein peak. Profiles of the two activities also coincided in diethylaminoethyl-cellulose chromatography and zonal centrifugation. These studies indicated that the esterase and the lyase were either complexed or similar molecular species. The former seems more probable because of the relatively high molecular weight. Both activities were most stable at pH 6.0. The esterase was inactivated rapidly at pH 5 or 7. Lyase preparations were freed of pectinesterase activity by heating for 30 min at 38 C and pH 7.0.

机译：在琼脂糖凝胶G-200上通过凝胶过滤色谱分别纯化了多发酵菌中的外半乳糖醛酸裂合酶和果胶酯酶，分别纯化了156倍和178倍。两种酶的活性在单个蛋白质峰中重合。在二乙氨基乙基纤维素色谱法和分区离心中，这两种活性的概况也一致。这些研究表明酯酶和裂解酶是复合的或相似的分子种类。由于分子量较高，前者似乎更有可能。两种活性在 p H 6.0时最稳定。酯酶在 p H 5或7处迅速失活。在38°C和 p H 7.0加热30分钟，裂解酶制剂没有果胶酯酶活性。 展开▼

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《Journal of bacteriology》 |1970年第1期|共7页

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L. Miller; J. D. Macmillan;
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1. Mode of Action of Pectic Enzymes II. Further Purification of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans [J] . L. Miller, J. D. Macmillan Journal of bacteriology . 1970,第1期

机译：果胶酶的作用方式II。从多发酵梭菌中进一步纯化外半乳糖醛酸裂合酶和果胶酯酶

2. Similarities in the Action Patterns of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans [J] . M. Lee, L. Miller, J. D. Macmillan Journal of bacteriology . 1970,第3期

机译：多发酵梭菌胞外半乳糖醛酸裂合酶和果胶酯酶作用方式的相似性

3. Investigations on inhibitors of human 17 alpha-hydroxylase-17,20-lyase and their interactions with the enzyme. Molecular modelling of 17 alpha-hydroxylase-17,20-lyase, Part II. [J] . Schappach A, Holtje HD Die Pharmazie . 2001,第11期

机译：人17α-羟化酶-17,20-裂解酶抑制剂及其与酶的相互作用的研究。 17α-羟化酶-17,20-裂合酶的分子模型，第二部分。

4. Characterization of Nanostructural Modifications Introduced into a Model Pectic Homogalacturonan by Esterases or Chemical Saponiflcation and Modeling of Enzyme Mode of Action [C] . Randall G. Cameron, Gary A. Luzio, Yang Kim, NSTI nanotechnology conference and expo;Nanotech conference expo;NSTI-Nanotech 2011;TechConnect world annual conference expo . 2011

机译：通过酯酶或化学皂化作用引入典型的半乳糖醛酸聚糖的纳米结构修饰的表征和酶作用模式的建模

5. Bacillus subtilis adenylosuccinate lyase: I. Identification of enzyme active site residues. II. Model system for elucidating the biochemistry of human acenylosuccinate lyase deficiency . [D] . Palenchar, Jennifer Brosius. 2003

机译：枯草芽孢杆菌腺苷琥珀酸裂解酶：I.酶活性位点残基的鉴定。二。模型系统阐明人类生化的酰基琥珀酸裂解酶缺乏。

6. Mode of Action of Pectic Enzymes II. Further Purification of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans [O] . L. Miller, J. D. Macmillan 1970

机译：果胶酶的作用方式II。从多发酵梭菌中进一步纯化外半乳糖醛酸裂合酶和果胶酯酶

7. Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product. [O] . Brooks, A D, He, S Y, Gold, S, 1990

机译：菊花欧文氏菌EC16的外聚半乳糖醛酸裂合酶结构基因的分子克隆和酶产物的表征。

8. Purification and Properties of Spore-Lytic Enzymes from Clostridium perfringens Type A Spores. [R] . Gomas, D. E., Labbe, R. G. 1985

机译：产气荚膜梭菌a型孢子孢子裂解酶的纯化及性质。

1. 拜氏梭菌Clostridium beijerinckii NCIMB 8052发酵玉米皮生产丁醇 [J] . 沈兆兵 ,刘晓洁 ,刘莉 . 生物技术进展 . 2014,第005期

2. 拜氏梭菌Clostridium beijerinckii NCIMB 8052在木薯发酵中耐高浓度丁醇的菌株筛选 [J] . 苏海锋 ,杨登峰 . 酿酒科技 . 2010,第009期

3. 由商品果胶酶制剂制备高纯果胶酯酶制剂的研究 [A] . 张跃 . 2007

1. Pectic enzyme preparations fortified with pectinesterase [P] . 外国专利： US2457560A . 1948-12-28

机译：果胶酯酶强化的果胶酶制剂

2. METHOD FOR MASS PRODUCTION AND PURIFICATION BIOCATALYST (ENZYME) FOR DECOMPOSING AROMATIC TOXIC ORGANIC COMPOUND, AND ENVIRONMENTALLY FRIENDLY NANOSTRUCTURE-BASED BIOCATALYST IMMOBILIZING LYASE PRODUCED BY SAME [P] . 外国专利： KR20140073634A . 2014-06-17

机译：大规模生产和纯化用于分解芳族有毒有机化合物的酶的方法，以及基于环境的，以纳米结构为基础的固定化生产的裂解酶的生物催化剂

3. method for preparation of protein hydrolysates of proteins by exposing to the action of an enzyme ii. [P] . 外国专利： NL139655B . 1973-09-17

机译：通过暴露于酶的作用制备蛋白质的蛋白质水解产物的方法ii。

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Mode of Action of Pectic Enzymes II. Further Purification of Exopolygalacturonate Lyase and Pectinesterase from Clostridium multifermentans

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