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首页> 外文期刊>Journal of bacteriology >Incorporation of 5-Diazouracil-2-14C into Ribonucleic Acids of Escherichia coli During Division Inhibition
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Incorporation of 5-Diazouracil-2-14C into Ribonucleic Acids of Escherichia coli During Division Inhibition

机译：在分区抑制过程中将5-重氮尿嘧啶-2-14C掺入大肠杆菌的核糖核酸中

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摘要

Radioactive diazouracil (DZU-2-14C) was rapidly incorporated into acid-insoluble material of Escherichia coli B. Isolated ribonucleic acid contained essentially all of the incorporated label, and this was solubilized by ribonucleases but not by deoxyribonuclease. A maximum of 45 to 50% of added label was incorporated at division-inhibitory and subinhibitory concentrations. Incorporation levels and filament-forming capacity were concomitantly depressed by preincubation of DZU in various medium components. The lower levels of incorporation brought about by preincubation were apparently related to an inherent instability or high reactivity of the DZU. The limit on incorporation of fresh DZU suggests the presence of geometrical isomers in the original DZU. The nature of these isomers and the reactivity of DZU are discussed in relation to its use in future cell division studies.
机译：将放射性重氮尿嘧啶（DZU- 2 - 14 C ）快速掺入大肠杆菌 B的酸不溶物中分离的核糖核酸基本上包含所有掺入的标记，并且其被核糖核酸酶而不是被脱氧核糖核酸酶溶解。在抑制分裂抑制和亚抑制浓度下，最多添加了45％至50％的添加标记。在各种培养基成分中预孵育DZU会同时降低掺入水平和长丝形成能力。预孵育引起的较低掺入水平显然与DZU固有的不稳定性或高反应性有关。新鲜DZU掺入的限制表明原始DZU中存在几何异构体。讨论了这些异构体的性质和DZU的反应性，并将其用于未来的细胞分裂研究中。

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