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首页> 外文期刊>Journal of bacteriology >Localization of glycogen synthetase during differentiation of presumptive cell types in Dictyostelium discoideum.
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Localization of glycogen synthetase during differentiation of presumptive cell types in Dictyostelium discoideum.

机译：盘基网柄菌的推测细胞类型分化过程中糖原合成酶的定位。

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Ultramicrochemical techniques were utilized to assay glycogen synthetase (EC 2.4.1.11) activity in cell samples of Dictyostelium discoideum as small as 0.01 mug (dry weight) in reaction volumes of 0.1 mul. The activity was assayed by an amplification procedure employing the enzymatic cycling of pyridine nucleotides. These techniques were used to determine the extent of localization of glycogen synthetase in the two cell types during differentiation of D. discoideum. Localization studies in developing spore cells revealed decreasing enzyme activity to the culmination stage. During this phase of development, the enzyme required the presence of soluble glycogen for activity. From culmination to sorocarp stage, enzyme activity increased and was independent of the soluble glycogen. In developing stalk cells, synthetase showed a decreasing gradient of activity. In sorocarps, the cells in the stalk apex showed synthetase activity similar to that of the spores. The cells at the bottom of the stalk had no detectable activity.
机译：超微化学技术被用于测定小至0.01马克杯（干重）的小球果盘基茎细胞样品中糖原合成酶（EC 2.4.1.11）活性，反应体积为0.1 mul。通过使用吡啶核苷酸的酶促循环的扩增程序来测定活性。这些技术被用来确定D. discoideum分化过程中两种细胞类型中糖原合成酶的定位程度。在发育中的孢子细胞中的定位研究表明，酶活性降低到高潮阶段。在此发育阶段，该酶需要可溶性糖原的存在才能发挥活性。从高潮到果皮阶段，酶活性增加，并且与可溶性糖原无关。在发育中的茎细胞中，合成酶显示出降低的活性梯度。在果皮中，茎尖中的细胞显示出与芽孢相似的合成酶活性。茎底部的细胞没有可检测的活性。

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