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首页> 外文期刊>Journal of bacteriology >Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens.
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Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens.

机译：糖原，透明质酸盐和其他一些多糖极大地增强了粘质沙雷氏菌的脂酶的形成。

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摘要

Among 21 different polysaccharides tested, 5 greatly enhanced the spontaneous and cyclic AMP-induced formation of exolipase: glycogen, hyaluronate, laminarin, pectin B, and gum arabic. These polysaccharides have in common the tendency to form highly ordered networks because of the branching or helical arrangement, or both, of their molecules. None of the polysaccharides could be utilized by the cells as the sole carbon source. Strong lipid extraction of four different polysaccharides did not reduce their exolipase-enhancing efficacy. At a constant cell density the stimulation of exolipase formation by various concentrations of glycogen followed saturation kinetics, suggesting a limited number of "sites" for the glycogen to act. The active principle present in a solution of pectin was destroyed by degradation (beta-elimination) of the polymer. Hyaluronate lost its exolipase-enhancing activity by exhaustive hydrolysis with hyaluronidase but was resistant to proteinase K. Exopolysaccharide, isolated from growth medium of Serratia marcescens SM-6, enhanced the exolipase formation as efficiently as hyaluronate. The results of this work are discussed mainly in terms of the "detachment hypothesis."
机译：在测试的21种不同多糖中，有5种极大地增强了自发性和环状AMP诱导的外脂酶的形成：糖原，透明质酸盐，层粘连蛋白，果胶B和阿拉伯胶。这些多糖由于其分子的分支或螺旋排列或两者共同具有形成高度有序网络的趋势。细胞不能将任何多糖用作唯一的碳源。对四种不同的多糖进行强力脂质提取并没有降低它们的外脂酶增强功效。在恒定的细胞密度下，各种浓度的糖原刺激脂肪酶形成的过程遵循饱和动力学，表明糖原发挥作用的“位点”数量有限。果胶溶液中存在的活性成分被聚合物降解（β-消除）破坏。透明质酸通过透明质酸酶的彻底水解而丧失了其增强外切酶的活性，但对蛋白酶K具有抗性。从粘质沙雷氏菌（Serratia marcescens SM-6）生长培养基中分离出的外多糖与透明质酸一样有效地增强了外切酶的形成。这项工作的结果主要根据“分离假设”进行讨论。

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