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首页> 外文期刊>Journal of bacteriology >Evidence for stable messenger ribonucleic acid during sporulation and enterotoxin synthesis by Clostridium perfringens type A.
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Evidence for stable messenger ribonucleic acid during sporulation and enterotoxin synthesis by Clostridium perfringens type A.

机译：A型产气荚膜梭状芽孢杆菌在孢子形成和肠毒素合成过程中稳定信使核糖核酸的证据。

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Stable messenger ribonucleic acid (mRNA) was shown to be involved in both enterotoxin synthesis and synthesis of other spore coat proteins in Clostridium perfringens. When used at a concentration that inhibited [14C]uracil incorporation, rifampin, a specific inhibitor of deoxyribonucleic acid-dependent RNA polymerase, prevented incorporation of a mixture of labeled amnoo acids by 3-h sporulating cells. At that time, enterotoxin protein was first detectable and cells were primarily at stage II or III of sporulation. When rifampin or streptolydigin was added to 5-h sporulating cells, which were primarily at stage IV or V and had significant toxin levels, incorporation of labeled amino acids continued through 30 min despite its presence. Rifampin also failed to prevent the specific synthesis of enterotoxin, a structural protein of the spore coat. The half-life of enterotoxin RNA was estimated to be at least 58 min. When cell extracts from 5-h sporulating cells that had been exposed to 3H-labeled amino acids for 10 min were subjected to electrophoresis on polyacrylamide gels and the gels were subsequently analyzed for radioactivity, two major peaks of radioactivity were obtained. The two peaks corresponded to enterotoxin and another spore coat protein(s). Similar results were obtained when the cells had been preincubated for 60 min with rifampin before label addition, indicating the functioning of stable mRNA.
机译：稳定的信使核糖核酸（mRNA）被证明与产气荚膜梭菌中肠毒素的合成和其他孢子外壳蛋白的合成有关。当以抑制[14C]尿嘧啶掺入的浓度使用时，利福平（一种依赖于脱氧核糖核酸的RNA聚合酶的特异性抑制剂）通过3小时的孢子形成细胞阻止了标记的壬酸混合物的掺入。那时，肠毒素蛋白是最先被检测到的，细胞主要处于孢子形成的第二阶段或第三阶段。当将利福平或链霉抗生物素蛋白添加到5小时的形成孢子的细胞中时，该细胞主要处于IV或V期，并具有明显的毒素水平，尽管存在，但标记氨基酸的掺入仍持续了30分钟。利福平也未能阻止肠毒素的特异性合成，肠毒素是孢子外壳的一种结构蛋白。肠毒素RNA的半衰期估计至少为58分钟。当将已经暴露于3H标记的氨基酸10分钟的5 h孢子形成细胞的细胞提取物在聚丙烯酰胺凝胶上进行电泳，然后分析凝胶的放射性时，得到两个主要的放射性峰。这两个峰对应于肠毒素和另一种孢子外壳蛋白。当在添加标记之前将细胞与利福平预孵育60分钟时，可获得相似的结果，表明稳定的mRNA的功能。

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