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首页> 外文期刊>Journal of bacteriology >Pyrimidine dimer excision in a Bacillus subtilis Uvr- mutant.
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Pyrimidine dimer excision in a Bacillus subtilis Uvr- mutant.

机译：枯草芽孢杆菌Uvr-突变体中的嘧啶二聚体切除。

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摘要

A technique which allows the measurement of small numbers of pyrimidine dimers in the deoxyribonucleic acid (DNA) of cells of Bacillus subtilis irradiated with ultraviolet light has been used to show that a strain mutant at the uvr-1 locus is able to excise pyrimidine dimers. Excision repair in this strain was slow, but incision may not be rate limiting because single-strand breaks in DNA accumulate under some conditions. Excision repair probably accounted for a liquid-holding recovery previously reported to occur in this strain. Recombinational exchange of pyrimidine dimers into newly replicated DNA was readily detected in uvr-1 cells, but this exchange did not account for more than a minor fraction of the dimers removed from parental DNA. Excision repair in the uvr-1 strain was inhibited by a drug which complexes DNA polymerase III with DNA gaps. This inhibition may be limited to a number of sites equal to the number of DNA polymerase III molecules, and it is inferred that large gaps are produced by excision of dimers. Because the uvr-1 mutation specifically interferes with excision of dimers at incision sites, it is concluded that the uvr-1 gene product may be an exonuclease which is essential for efficient dimer excision.
机译：已经使用一种可以测量用紫外线照射的枯草芽孢杆菌细胞的脱氧核糖核酸（DNA）中少量嘧啶二聚体的技术，表明在uvr-1位点的菌株突变体能够切除嘧啶二聚体。该菌株的切除修复很慢，但是切开可能不是速率限制，因为在某些情况下DNA的单链断裂会积累。切除修复可能是先前报道的该菌株中持液量恢复的原因。在uvr-1细胞中可以很容易地检测到嘧啶二聚体向新复制的DNA的重组交换，但是这种交换所占的比重不超过从亲本DNA中去除的二聚体的一小部分。 uvr-1菌株的切除修复被一种将DNA聚合酶III与DNA缺口复合的药物抑制。该抑制作用可被限制在与DNA聚合酶III分子的数目相等的位点的数目上，并且推断通过二聚体的切除会产生大的缺口。由于uvr-1突变会特别干扰切开位点的二聚体切除，因此可以得出结论，uvr-1基因产物可能是核酸外切酶，对于有效的二聚体切除至关重要。

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