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首页> 外文期刊>Journal of bacteriology >Characterization of the carbohydrate component of fraction I in the Neurospora crassa cell wall.
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Characterization of the carbohydrate component of fraction I in the Neurospora crassa cell wall.

机译：刻纹夜蛾细胞壁中I部分碳水化合物成分的表征。

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The carbohydrate portion of fraction I of the Neurospora crassa cell wall has been analyzed for sugar composition by gas-liquid chromatography and colorimetric methods. The analysis was performed comparatively in a wild-type strain (RL 3-8A) and three morphological mutants: scumbo (FGSC 49), peak-2a (a mutant known to be allelic to biscuit), and ragged (FGSC 296). Fraction I of all strains studied contains glucose, mannose, and galactose as the main sugars. Uronic acids and amino sugars are also present in small amounts. The glycosidic linkages binding the neutral sugars were analyzed by Lindberg's combined gas chromatography-mass spectrometry techniques for identification of the partially methylated alditol acitate sugar derivatives. The main polymeric portion of fraction I seems to be a linear glucan with the glucose residues linked by 1 leads to 3 and 1 leads to 4 bonds. A mannan portion with a branched configuration is also present, with galactose as the sugar residue which serves as branches in the molecule(s). The branched mannan portion appears to increase in amount in correlation with more drastic morphological changes of the mycelia. In this respect, the mutant ragged has the lowest mycelial growth rate and the largest amount of mannan. The importance of the polysaccharide structure of fraction I on the colonial morphology of the mycelia is discussed.
机译：已通过气液色谱法和比色法分析了神经孢霉细胞壁I部分的碳水化合物部分的糖组成。在野生型菌株（RL 3-8A）和三个形态突变体中进行了比较分析：浮渣（FGSC 49），peak-2a（已知是饼干等位基因的突变体）和衣衫（的（FGSC 296）。研究的所有菌株的馏分I均含有葡萄糖，甘露糖和半乳糖作为主要糖分。糖醛酸和氨基糖也少量存在。通过Lindberg组合气相色谱-质谱技术分析结合中性糖的糖苷键，以鉴定部分甲基化的糖醇醛糖衍生物。组分I的主要聚合物部分似乎是直链葡聚糖，其中葡萄糖残基通过1个键连接至3个，并且通过1个键连接4个键。还存在具有分支构型的甘露聚糖部分，其中半乳糖作为糖残基，其在分子中充当分支。分支的甘露聚糖部分的数量似乎与菌丝体形态的急剧变化相关。在这方面，衣衫mutant的突变体具有最低的菌丝体生长速率和最大的甘露聚糖量。讨论了级分I的多糖结构对菌丝体殖民地形态的重要性。

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