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首页> 外文期刊>Journal of bacteriology >Escherichia coli K-12 mutants that allow transport of maltose via the beta-galactoside transport system.
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Escherichia coli K-12 mutants that allow transport of maltose via the beta-galactoside transport system.

机译：允许通过β-半乳糖苷转运系统转运麦芽糖的大肠杆菌K-12突变体。

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We have isolated mutants of Escherichia coli that have an altered beta-galactoside transport system. This altered transport system is able to transport a sugar, maltose, that the wild-type beta-galactoside transport system is unable to transport. The mutation that alters the specificity of the transport system is in the lacY gene, and we refer to the allele as lacYmal. The lacYmal allele was detected originally in strains in which the lac genes were fused to the malF gene. Thus, as a result of gene fusion and isolation of the lacYmal mutation, a new transport system was evolved with regulatory properties and specificity similar to those of the original maltose transport system. Maltose transport via the lacYmal gene product is independent of all of the normal maltose transport system components. The altered transport system shows a higher affinity than the wild-type transport system for two normal substrates of the beta-galactoside transport system, thiomethyl-beta-D-galactoside and o-nitrophenyl-beta-D-galactoside.
机译：我们已分离出具有改变的β-半乳糖苷转运系统的大肠杆菌突变体。这种改变的运输系统能够运输野生型β-半乳糖苷运输系统无法运输的糖，麦芽糖。改变转运系统特异性的突变在lacY基因中，我们将等位基因称为lacYmal。 lacYmal等位基因最初是在lac基因与malF基因融合的菌株中检测到的。因此，由于基因融合和lacYmal突变的分离，一个新的运输系统得以发展，其调控特性和特异性与原始麦芽糖运输系统相似。通过lacYmal基因产物的麦芽糖运输与所有正常麦芽糖运输系统组分无关。改变的转运系统对β-半乳糖苷转运系统的两种正常底物，硫代甲基-β-D-半乳糖苷和邻硝基苯基-β-D-半乳糖苷显示出比野生型转运系统更高的亲和力。

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