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首页> 外文期刊>Journal of bacteriology >Induction of cellulolytic enzymes in Trichoderma reesei by sophorose.
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Induction of cellulolytic enzymes in Trichoderma reesei by sophorose.

机译：槐糖浆诱导里氏木霉中的纤维素分解酶。

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摘要

Sophorose (2-O-beta-glucopyranosyl-D-glucose) induces carboxymethyl cellulase in Trichoderma reesei QM6a mycelium with 1.5 to 2 h. The induction response to sophorose concentration, although complicated by the metabolism of sophorose, shows saturation kinetics. Most of the cellulase appears after most of the sophorose has been taken up, but the presence of an inducer is required to maintain cellulase synthesis because enzyme production ceases after separation of the mycelium from the induction medium. Cellulase appears simultaneously in the medium and in the mycelium, and no appreciable levels accumulate in the mycelium. Response to pH suggest either that synthesis and secretion of the enzyme are closely associated or concurrent events affected by surface interactions with the medium. Effects of temperature and pH on cellulase induction by sophorose are similar to those reported for induction by cellulose. The kinetics of absorption by mycelium differs from that of other beta-linked saccharides and glucose, the uptake of sophorose being much slower. Under our cultural conditions, sophorose appears to induce an incomplete array of cellulase enzymes, as indicated by enzymatic and electrophoretic studies.
机译：槐糖（2-O-β-吡喃葡萄糖基-D-葡萄糖）在1.5至2小时内诱导里氏木霉QM6a菌丝体中的羧甲基纤维素酶。对槐糖浓度的诱导反应，尽管因槐糖的代谢而复杂化，但表现出饱和动力学。大部分纤维素酶被吸收后，大多数纤维素酶出现，但是需要诱导剂的存在来维持纤维素酶的合成，因为在从诱导培养基中分离菌丝体后酶的生产停止了。纤维素酶同时出现在培养基和菌丝体中，菌丝体中没有明显的水平积累。对pH的反应表明，酶的合成和分泌密切相关或同时发生，并受与培养基的表面相互作用影响。温度和pH对槐糖诱导纤维素酶的影响与报道的纤维素诱导作用相似。菌丝体的吸收动力学不同于其他β-连接的糖类和葡萄糖，其吸收慢得多。在我们的文化条件下，如酶和电泳研究所示，槐糖似乎诱导了不完整的纤维素酶阵列。

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