...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Regulation of Tryptophan Pyrrolase Activity in Xanthomonas pruni
【24h】

Regulation of Tryptophan Pyrrolase Activity in Xanthomonas pruni

机译：黄单胞菌色氨酸吡咯酶活性的调节

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tryptophan pyrrolase was studied in partially purified extracts of Xanthomonas pruni. The dialyzed enzyme required both heme and ascorbate for maximal activity. Other reducing agents were able to substitute for ascorbate. Protoporphyrin competed with heme for the enzyme, suggesting that the native enzyme is a hemoprotein. The enzyme exhibited sigmoid saturation kinetics. Reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), nicotinic acid mononucleotide, and anthranilic acid enhanced the sigmoid kinetics and presumably bound to allosteric sites on the enzyme. The sigmoid kinetics were diminished in the presence of α-methyltryptophan. NAD, NADP, nicotinic acid, nicotinamide, nicotinamide mononucleotide, and several other related compounds were without effect on the activity of the enzyme. These data indicate that the activity of the enzyme is under feedback regulation by the ultimate end products of the pathway leading to NAD biosynthesis, as well as by certain intermediates of this pathway.
机译：在部分纯化的黄单胞菌提取物中研究了色氨酸吡咯酶。透析酶需要血红素和抗坏血酸才能发挥最大活性。其他还原剂能够代替抗坏血酸。原卟啉与血红素竞争该酶，表明天然酶是血红蛋白。该酶表现出S形饱和动力学。减少的烟酰胺腺嘌呤二核苷酸（NADH），减少的烟酰胺腺嘌呤二核苷酸磷酸（NADPH），烟酸单核苷酸和邻氨基苯甲酸增强了乙状结肠动力学，并可能与酶的变构位点结合。在存在α-甲基色氨酸的情况下，乙状结肠动力学减弱。 NAD，NADP，烟酸，烟酰胺，烟酰胺单核苷酸和其他几种相关化合物对酶的活性没有影响。这些数据表明，酶的活性受到导致NAD生物合成的途径的最终产物以及该途径的某些中间体的反馈调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号