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首页> 外文期刊>Journal of bacteriology >Screening for amino acid pool mutants of Neurospora and yeasts: replica-printing technique.
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Screening for amino acid pool mutants of Neurospora and yeasts: replica-printing technique.

机译:筛选Neurospora和酵母的氨基酸库突变体:复制打印技术。

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摘要

A replica-printing technique has been developed for permeabilized colonies of Neurospora and yeast by which endogenous amino acid pools are visualized. By the use of cation-exchange paper, the method has detected Neurospora mutants unable to store the large pools of basic amino acids characteristic of wild type. Mutants of this sort were sought to study the function of the fungal vacuole.
机译:已经开发了用于神经孢菌属和酵母的透化菌落的复制品印刷技术,通过该技术可以看到内源性氨基酸库。通过使用阳离子交换纸,该方法检测到Neurospora突变体无法存储大量野生型特征性碱性氨基酸。寻找这种突变体以研究真菌液泡的功能。

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