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首页> 外文期刊>Journal of bacteriology >Plasmid Control of 6-Aminohexanoic Acid Cyclic Dimer Degradation Enzymes of Flavobacterium sp. K172
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Plasmid Control of 6-Aminohexanoic Acid Cyclic Dimer Degradation Enzymes of Flavobacterium sp. K172

机译：黄杆菌sp.6-氨基己酸环二聚体降解酶的质粒控制。 K172

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Flavobacterium sp. K172, which is able to grow on 6-aminohexanoic acid cyclic dimer as the sole source of carbon and nitrogen, and plasmid control of the responsible enzymes, 6-aminohexanoic acid cyclic dimer hydrolase and 6-aminohexanoic acid linear oligomer hydrolase, were studied. The wild strain of K172 harbors three kinds of plasmid, pOAD1 (26.2 megadaltons), pOAD2 (28.8 megadaltons), and pOAD3 (37.2 megadaltons). The wild strain K172 was readily cured of its ability to grow on the cyclic dimer by mitomycin C, and the cyclic dimer hydrolase could not be detected either as catalytic activity or by antibody precipitation. No reversion of the cured strains was detected. pOAD2 was not detected in every cured strain tested but was restored in a transformant. The transformant recovered both of the enzyme activities, and the cyclic dimer hydrolase of the transformant was immunologically identical with that of the wild strain. All of the strains tested, including the wild, cured, and transformant ones, possessed identical pOAD3 irrespective of the metabolizing activity. Some of the cured strains possessed pOAD1 identical with the wild strain, but the others harbored plasmids with partially altered structures which were likely to be derived from pOAD1 by genetic rearrangements such as deletion, insertion, or substitution. These results suggested that the genes of the enzymes were borne on pOAD2.
机译：黄杆菌 sp。研究了能够在6-氨基己酸环状二聚体上作为碳和氮的唯一来源生长的K172，以及负责酶，6-氨基己酸环状二聚体水解酶和6-氨基己酸线性低聚物水解酶的质粒控制。 K172的野生株带有三种质粒，pOAD1（26.2兆达尔顿），pOAD2（28.8兆达尔顿）和pOAD3（37.2兆达尔顿）。通过丝裂霉素C，野生菌株K172易于在环状二聚体上生长，并且不能通过催化活性或抗体沉淀检测到环状二聚体水解酶。没有检测到固化菌株的回复。在每个测试的治愈菌株中均未检测到pOAD2，但已在转化体中将其还原。该转化体恢复了这两种酶的活性，并且该转化体的环状二聚体水解酶在免疫学上与野生株相同。所测试的所有菌株，包括野生，治愈和转化菌株，都具有相同的pOAD3，而与代谢活性无关。一些治愈的菌株具有与野生菌株相同的pOAD1，但其他菌株则带有结构部分改变的质粒，这些质粒可能是通过基因重排（例如缺失，插入或取代）从pOAD1衍生而来的。这些结果表明，酶的基因被携带在pOAD2上。

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