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首页> 外文期刊>Journal of bacteriology >Control of Plasmid R1 Replication: Functions Involved in Replication, Copy Number Control, Incompatibility, and Switch-off of Replication
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Control of Plasmid R1 Replication: Functions Involved in Replication, Copy Number Control, Incompatibility, and Switch-off of Replication

机译：质粒R1复制的控制：涉及复制，拷贝数控制，不兼容和复制关闭的功能

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A small derivative of plasmid R1 was used to integratively suppress a chromosomal dnaA(Ts) mutation. The strain obtained grew normally at 42°C. The integratively suppressed strain was used as recipient for various plasmid R1 derivatives. Plasmid R1 and miniplasmid derivatives of R1 could be established in the strain that carried an integrated R1 replicon, but they were rapidly lost during growth. However, plasmids also carrying ColE1 replication functions were almost completely stably inherited. The integratively suppressed strain therefore allows the establishment of bacteria diploid with respect to plasmid R1 and forms a useful and sensitive system for studies of interaction between plasmid R1 replication functions. Several of the chimeric plasmids caused inhibition of growth at high temperatures. All plasmids that inhibited growth carried one particular PstI fragment from plasmid R1 (the PstI F fragment), and in all cases the growth inhibition could be ascribed to repression of initiation of chromosome replication at 42°C, i.e., they carry a trans-acting switch-off function. Furthermore, the analogous PstI fragments from different copy mutants of plasmid R1 were analyzed similarly, and one mutant was found to lack the switch-off function. The different chimeric plasmids were also tested for their incompatibility properties. All plasmids that carried the switch-off function (and no other plasmids) also carried R1 incompatibility gene(s). Since the PstI F fragment, which is present on all these plasmids, is very small (0.35 × 106), it is suggested that the switch-off regulation of replication (by an inhibitor), incompatibility, and copy number control are governed by the same gene.
机译：质粒R1的一个小衍生物用于整合抑制染色体 dnaA （Ts）突变。获得的菌株在42℃下正常生长。整合抑制的菌株用作各种质粒R1衍生物的受体。可以在携带整合的R1复制子的菌株中建立质粒R1和R1的微质粒衍生物，但是它们在生长过程中迅速丢失。然而，也具有ColE1复制功能的质粒几乎完全稳定地遗传。因此，被整体抑制的菌株允许相对于质粒R1建立细菌二倍体，并形成用于研究质粒R1复制功能之间相互作用的有用和敏感的系统。几种嵌合质粒引起高温下的生长抑制。所有抑制生长的质粒都带有一个来自质粒R1的特定 Pst I片段（ Pst IF片段），在所有情况下，生长抑制都可归因于抑制启动染色体在42°C复制，即它们具有 trans 起作用的关闭功能。此外，相似地分析了来自质粒R1的不同拷贝突变体的类似的 Pst I片段，发现一个突变体缺乏关闭功能。还测试了不同的嵌合质粒的不相容性。所有具有关闭功能的质粒（无其他质粒）也具有R1不相容基因。由于所有这些质粒上存在的 Pst IF片段非常小（0.35×10 6 ），因此建议关闭复制调控（抑制剂），不相容性和拷贝数控制由同一基因控制。

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