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首页> 外文期刊>Journal of bacteriology >Characterization of amber and ochre suppressors in Salmonella typhimurium.
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Characterization of amber and ochre suppressors in Salmonella typhimurium.

机译:鼠伤寒沙门氏菌中琥珀和o石抑制剂的表征。

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摘要

Amber and ochre suppressor mutations in Salmonella typhimurium were selected. The amino acid insertions directed by the suppressors were inferred from suppression patterns of Escherichia coli lacI amber mutations. These amber mutations only respond to nonsense suppressors that direct the insertion of particular amino acids. Four Salmonella amber suppressors characterized insert serine, glutamine, tyrosine, and (probably) leucine. Of the three ochre suppressors characterized, two direct the insertion of tyrosine and one directs that of lysine. Of the three amber and two ochre suppressors which have been mapped by phage P22 cotransduction, all are located in the same relative position on the Salmonella map as the analogous E. coli suppressors are on the E. coli map.
机译:选择鼠伤寒沙门氏菌中的琥珀色和o色抑制基因突变。抑制子指导的氨基酸插入是从大肠杆菌lacI琥珀色突变的抑制模式推断出来的。这些琥珀色突变仅对指导特定氨基酸插入的废话抑制剂产生反应。四种沙门氏菌琥珀抑制剂的特征是插入丝氨酸,谷氨酰胺,酪氨酸和(可能)亮氨酸。在三种特征的o石抑制剂中,两种指示酪氨酸的插入,一种指示赖氨酸的插入。在通过噬菌体P22共转导定位的三个琥珀色和两个o色抑制剂中,与类似的大肠杆菌抑制剂在大肠杆菌图谱上一样,它们在沙门氏菌图中均位于相同的相对位置。

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