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首页> 外文期刊>Journal of bacteriology >Bacillus cereus autolytic endoglucosaminidase active on cell wall peptidoglycan with N-unsubstituted glucosamine residues.
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Bacillus cereus autolytic endoglucosaminidase active on cell wall peptidoglycan with N-unsubstituted glucosamine residues.

机译：蜡状芽孢杆菌自溶性内酰胺酶对具有N-未取代的葡糖胺残基的细胞壁肽聚糖具有活性。

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摘要

An autolytic glycosidase from a lysozyme-resistant strain of Bacillus cereus capable of cleaving the glycosidic linkages of N-unsubstituted glucosamine in the cell wall peptidoglycan was studied. This glycosidase activity, together with N-acetylmuramyl-L-alanine amidase activity, was found in an autolytic enzyme preparation obtained from the 20,000 x g precipitate fraction by means of autolysis followed by ammonium sulfate fractionation. The major saccharide fragments resulting from digestion of the untreated, non-N-acetylated, cell wall peptidoglycan of B. cereus with the autolytic enzyme preparation were identified as N-acetylmuramyl-glucosamine and its dimer. The peptidoglycan N-acetylated with acetic anhydride could also be digested with the same enzyme preparation, giving N-acetylmuramyl-N-acetylglucosamine and its dimer as the major saccharide fragments.
机译：研究了一种来自蜡样芽孢杆菌的耐溶菌酶菌株的自溶糖苷酶，该菌株能够裂解细胞壁肽聚糖中N-未取代的氨基葡萄糖的糖苷键。该糖苷酶活性与N-乙酰基村酰基-L-丙氨酸酰胺酶活性一起在自溶酶制剂中发现，该自溶酶制剂是通过自溶然后硫酸铵分级分离而从20,000×g沉淀物级分获得的。用自溶酶制剂消化蜡状芽孢杆菌的未处理的，未N-乙酰化的细胞壁肽聚糖所产生的主要糖片段被鉴定为N-乙酰村酰-葡糖胺及其二聚体。也可以用相同的酶制剂消化用乙酸酐N-乙酰化的肽聚糖，得到N-乙酰村酰-N-乙酰葡糖胺及其二聚体作为主要糖片段。

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