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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Molecular Profile of Emerging Multidrug Resistant Klebsiella pneumoniae Clinical Isolates from Southern India
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Molecular Profile of Emerging Multidrug Resistant Klebsiella pneumoniae Clinical Isolates from Southern India

机译：印度南部新兴的多药耐药性肺炎克雷伯氏菌的分子特征

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Multidrug Resistance (MDR) in Klebsiella pneumoniae isolates is an increasingly recognised threat to hospital infection control. It is known to produce a wide array of cephalosporinase and carbapenemase enzymes.Aim: This study was done to determine the prevalence of MDR in K. pneumoniae with phenotypic and genotypic characterisation of Extended Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance mechanisms.Materials and Methods: Out of 562 K. pneumoniae isolates recovered during November 2014 to June 2015 in our tertiary care hospital in Pondicherry, 117 MDR strains were phenotypically analysed for presence of various types of beta lactamases and carbapenemases by ceftazidime-clavulanic acid combined disc test, AmpC disc test, Modified Hodge's test and meropenem-EDTA combined disc test. These isolates were further screened for ESBL (bla CTX-M, bla SHV-1, bla TEM) and Carbapenemase genes (bla NDM-1, bla IMP-1, bla VIM-2, bla SIM-1 and bla KPC) by multiplex PCR.Results: Prevalence of MDR strains of K. pneumoniae was 20.8%. Out of 117 MDR K. pneumoniae , ESBL, AmpC and MBL mediated resistance was identified by phenotypic method in 91, 27 and 16 isolates respectively. Among the ESBL and MBL genes, bla CTX-M (60.6%), bla SHV-1 (69%), bla NDM-1 (33%) and bla IMP-1 (9%) genes were detected. Co-production of multiple enzymes was observed in 32% isolates.Conclusion: Beta-lactam hydrolysing enzymes are prevalent among MDR K. pneumoniae stains in our region. Co-expression of ESBL and MBL genes are found in a large proportion of clinical isolates of K. pneumoniae .
机译：肺炎克雷伯菌的多药耐药性（MDR）是医院感染控制日益受到认可的威胁。已知会产生各种各样的头孢菌素酶和碳青霉烯酶。目的：进行这项研究以确定MDR在K中的患病率。肺炎支原体具有表型和基因型特征的超广谱β-内酰胺酶（ESBL），AmpC和碳青霉烯酶介导的耐药机制。材料与方法：出自562 K。在2014年11月至2015年6月于本地治里的三级医院中分离出的肺炎分离株中，通过头孢他啶-克拉维酸联合圆盘检验，AmpC圆盘检验，改良的Hodge检验和美罗培南-EDTA联合椎​​间盘测试。进一步筛选这些分离物的ESBL（bla CTX-M，bla SHV-1，bla TEM）和碳青霉烯酶基因（bla NDM-1，bla IMP-1，通过多重PCR进行 bla VIM-2， bla SIM-1和 bla KPC）。结果： K的MDR菌株普遍存在。肺炎为20.8％。在117 MDR K中。通过表型方法分别在91、27和16个分离物中鉴定出肺炎，ESBL，AmpC和MBL介导的抗药性。在ESBL和MBL基因中，bla CTX-M（60.6％），bla SHV-1（69％），bla NDM-1（33％）和bla IMP-1 （9％）基因被检测到。在32％的分离物中观察到多种酶的共同产生。结论：β-内酰胺水解酶在MDR K中普遍存在。我们地区出现肺炎。在很大比例的临床分离株中发现了ESBL和MBL基因的共表达。肺炎。

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