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首页> 外文期刊>Journal of bacteriology >Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores.
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Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores.

机译：营养学上确定的绿链霉菌孢子萌发条件。

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Spores of Streptomyces viridochromogenes were removed from the surface of solid media with glass beads and suspended in a buffer-detergent solution. Addition of yeast extract and glucose resulted in rapid loss of refractility of the spores. Appearance of germ tubes followed. Germination was accompanied by a decrease in the optical density (OD) of the suspension. The OD decrease was used as an assay for germination. A defined germination medium (DGM) comprised of L-alanine, L-glutamic acid, adenosine, para-aminobenzoic acid, and calcium and magnesium ions provided a germination rate nearly equal to that of complex media. The germination rate was essentially the same if D-alanine and D-glutamate replaced the L-isomers. The optimum pH and temperature for germination were 7.0 and 35 C. Germination was absolutely dependent on the presence of CO2. Spores harvested after growth for longer periods than the usual time (10 days) became less germinable in DGM. The same was observed for spores grown at 37 C as compared with 30 C. Spores incubated in DGM for various time periods before being transferred to a buffer solution did not continue to germinate. Spores harvested after growth of eight species of Streptomyces did not show a decrease in OD when incubated in yeast extract medium. Another strain of S. viridochromogenes did exhibit an OD decrease in the medium. Comparative properties of spores of streptomycetes, fungi, and bacilli are discussed.
机译：用玻璃珠从固体培养基的表面除去绿链霉菌的孢子，并将其悬浮在缓冲剂溶液中。酵母提取物和葡萄糖的添加导致孢子的折射性快速丧失。随后出现胚芽管。萌发伴随着悬浮液的光密度（OD）降低。 OD降低用作发芽测定。由L-丙氨酸，L-谷氨酸，腺苷，对氨基苯甲酸，钙和镁离子组成的限定发芽培养基（DGM）提供的发芽率几乎与复杂培养基相同。如果D-丙氨酸和D-谷氨酸代替L-异构体，则发芽率基本相同。萌发的最佳pH和温度为7.0和35C。发芽完全取决于CO2的存在。在比正常时间（10天）更长的生长时间后收获的孢子在DGM中的发芽率降低。与在30°C时相比，在37°C下生长的孢子也观察到了同样的情况。在DGM中温育的孢子在转移到缓冲液中之前未持续发芽。在酵母提取液中孵育后，八种链霉菌生长后收获的孢子的OD并未降低。病毒链球菌的另一种菌株在培养基中的OD确实降低了。讨论了链霉菌，真菌和杆菌的孢子的比较特性。

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