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首页> 外文期刊>Journal of bacteriology >Kinetic characterization of the two phosphate uptake systems in the fungus Neurospora crassa.
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Kinetic characterization of the two phosphate uptake systems in the fungus Neurospora crassa.

机译：真菌Neurospora crassa中两个磷酸盐吸收系统的动力学表征。

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摘要

The kinetics of phosphate uptake by exponentially growing Neurospora crassa were studied to determine the nature of the differences in uptake activity associated with growth at different external phosphate concentrations. Conidia, grown in liquid medium containing either 10 mM or 50 micronM phosphate, were harvested, and their phosphate uptake ability was measured. Initial experiments, where uptake was examined over a narrow concentration range near that of the growth medium, indicated the presence of a low-affintiy (high Km) system in germlings from 50 micronM phosphate. Uptake by each system was energy dependent and sensitive to inhibitors of membrane function. No efflux of phosphate or phosphorus-containing compounds could be detected. When examined over a wide concentration range, uptake was consistent with the simultaneous operation of low- and high-affinity systems in both types of germlings. The Vmax estimates for the two systems were higher in germlings from 50 micronM phosphate than for the corresponding systems in germlings from 10 mM phosphate. The Km of the high-affinity system was the same in both types of germlings, whereas the Km of the low-affinity system in germlings from 10 mM phosphate was about three three times that of the system in germlings from 50 micronM phosphate.
机译：研究了指数增长的神经孢霉的磷酸盐吸收动力学，以确定在不同外部磷酸盐浓度下与生长相关的吸收活性差异的性质。收获在含有10 mM或50 microM磷酸盐的液体培养基中生长的分生孢子，并测量其对磷酸盐的吸收能力。最初的实验在接近生长培养基的狭窄浓度范围内对摄取进行了检查，结果表明从50微米磷酸盐中的幼苗中存在低亲和力（高Km）系统。每个系统的吸收都是能量依赖的，并且对膜功能的抑制剂敏感。没有检测到磷酸盐或含磷化合物的流出。在较宽的浓度范围内进行检查时，两种苗种的吸收均与低亲和力系统和高亲和力系统的同时运行相一致。在50微米磷酸盐的幼苗中，这两个系统的Vmax估计值比在10毫米磷酸盐的幼苗中的相应系统的Vmax更高。在两种类型的幼苗中，高亲和力系统的Km均相同，而在10 mM磷酸盐的幼苗中，低亲和力系统的Km约为在50 microM磷酸盐的幼苗中的低三倍。

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