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首页> 外文期刊>Journal of bacteriology >Distribution of coenzyme F420 and properties of its hydrolytic fragments.
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Distribution of coenzyme F420 and properties of its hydrolytic fragments.

机译：辅酶F420的分布及其水解片段的性质。

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摘要

The ability of hydrolytic products of coenzyme F420 to substitute for F420 in the hydrogenase and nicotinamide adenine dinucleotide phosphate-liniked hydrogenase systems of Methanobacterium strain M.o.H. was kinetically determined. The nicotinamide adenine dinucleotide phosphate-linked hydrogenase system was employed to quantitate the levels of F420 in a number of methanogenic bacteria as well as in some nonmethanogens. Methanobacterium ruminantium and Methanosarcina barkeri contained low levels of F420, whereas other methanogens tested contained high levels (100 to 400 mg/kg of cells). F420 from six of the seven methanogens was tested by thin-layer electrophoresis and was found to be electrophoretically identical to that purified from Methanobacterium strain M.o.H. The only exception was M. barkeri, which contained a more electronegative derivative of F420. Acetobacterium woodii, Escherichia coli, and yeast extract contained no compounds able to substitute for F420 in the nicotinamide adenine dinucleotide phosphate-linked hydrogenase system.
机译：辅酶F420的水解产物在甲烷杆菌M.o.H.的氢化酶和烟酰胺腺嘌呤二核苷酸磷酸酯化的氢化酶系统中替代F420的能力。是动力学确定的。烟酰胺腺嘌呤二核苷酸磷酸连接的氢化酶系统用于定量许多产甲烷细菌和某些非甲烷类细菌中F420的水平。反刍类甲烷菌和巴氏甲烷八叠球菌的F420含量较低，而其他产甲烷菌的含量较高（100至400 mg / kg细胞）。通过薄层电泳对来自七个产甲烷菌中六个中的F420进行了测试，发现与从甲烷杆菌M.o.H.唯一的例外是巴氏甲烷八叠球菌，其中含有F420的更多负电性衍生物。木本醋杆菌，大肠杆菌和酵母提取物在烟酰胺腺嘌呤二核苷酸磷酸连接的氢化酶系统中不含能够替代F420的化合物。

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