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首页> 外文期刊>Journal of bacteriology >Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus.
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Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus.

机译：溶葡萄球菌溶葡萄球菌内肽酶的产生与细胞壁组成的关系。

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摘要

Mutants of Staphylococcus staphylolyticus incapable of producing an extracellular staphylolytic glycylglycine endopeptidase were isolated and found to have cells in the population susceptible to lysis by this enzyme, as did the wild-type organism under conditions in which the endopeptidase was not produced. These results suggest that cultures of this organism normally contain a heterogeneous population of cells with regard to cell wall composition and susceptibility to the enzyme. Production of the endopeptidase appears to act as a selective pressure which removes the susceptible cells in the population as the enzyme appears in the medium. A comparison of the peptidoglycan of the wild-type organism grown under conditions in which the endopeptidase was produced with that of this organism grown under nonproducing conditions and with those of endopeptidase-less mutants showed that in the presence of the endopeptidase the cell population had peptidoglycan with shorter peptide cross bridges and a greater percentage of serine in these cross bridges than was found in cells grown in the absence of the enzyme. The inability of the endopeptidase to hydrolyze glycylserine and serylglycine peptide bonds suggests that at least part of the resistance this organism has to the endopeptidase is due to relative amounts of serine found in the peptide cross bridges of some cells in the population.
机译：与不能产生内肽酶的野生型生物体一样，分离出不能产生细胞外葡萄球菌型糖基甘氨酸内肽酶的葡萄球菌突变体，发现其种群中的细胞易于被该酶裂解。这些结果表明，就细胞壁组成和对酶的敏感性而言，该生物的培养物通常含有异质细胞群。内肽酶的产生似乎起选择压力的作用，因为该酶出现在培养基中，从而去除了群体中的易感细胞。将在其中产生内肽酶的条件下生长的野生型生物体的肽聚糖与在非生产条件下生长的该生物体的肽聚糖和与无内肽酶的突变体的肽聚糖进行比较，表明在存在内肽酶的情况下，细胞群具有肽聚糖与在不存在酶的情况下生长的细胞中发现的相比，具有较短的肽交叉桥和在这些交叉桥中的丝氨酸百分比更高的肽。内肽酶不能水解甘氨酰丝氨酸和丝氨酰甘氨酸肽键，这表明该生物体对内肽酶的至少部分抗性是由于在种群中某些细胞的肽桥中发现的丝氨酸相对含量所致。

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