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首页> 外文期刊>Journal of bacteriology >Effect of carbamoyl phosphate on nitrogenase in Anabaena cylindrica Lemm.
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Effect of carbamoyl phosphate on nitrogenase in Anabaena cylindrica Lemm.

机译：氨基甲酸酯磷酸酯对An鱼腥藻中固氮酶的影响。

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摘要

Carbamoyl phosphate inhibited acetylene reduction by whole cells and cell-free extracts of Anabaena cylindrica. Higher levels of both endogenous carbamoyl phosphate and carbamoyl phosphate synthase activity were present in NH4+-grown cells (in which acetylene reduction was absent) than in N2-grown cells (in which acetylene reduction was present). However, inhibition of acetylene reduction was observed also with cyanate, the main initial decomposition product under the conditions used. It is concluded that carbamoyl phosphate or one of its metabolites may act as a physiological regulator of both nitrogenase activity and synthesis, but caution must be used in interpreting effects observed several hours after the addition of carbamoyl phosphate, because the effects may be due to cyanate.
机译：氨基甲酸酯磷酸抑制鱼腥藻全细胞和无细胞提取物的乙炔还原。在NH4 +生长的细胞（不存在乙炔还原反应）中，内源性氨基甲酰磷酸和氨基甲酰磷酸合酶活性水平高于在N2生长的细胞（存在乙炔还原反应）中。但是，在使用的条件下，主要的初始分解产物氰酸盐也观察到了乙炔还原的抑制作用。结论是，氨基甲酸酯磷酸酯或其代谢物之一可以充当固氮酶活性和合成的生理调节剂，但是在解释添加氨基甲酸酯磷酸酯几个小时后观察到的作用时必须谨慎，因为这种作用可能是由于氰酸盐引起的。 。

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