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首页> 外文期刊>Journal of bacteriology >Light-induced, carrier-mediated transport of tetracycline by Rhodopseudomonas sphaeroides.
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Light-induced, carrier-mediated transport of tetracycline by Rhodopseudomonas sphaeroides.

机译：球形红假单胞菌的光诱导，载体介导的四环素转运。

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摘要

Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated, with a Km of 25 mM. Chlorotetracycline competitively inhibited tetracycline transport. The transport was energy dependent. Efflux occurred during the influx process, and an energy-requiring steady state was reached when influx balanced efflux. Transport was inhibited by metabolic inhibitors such as antimycin A, cyanide, and iodoacetate. Proton conductors such as carbonylcyanide m-chlorophenyl hydrazone were strongly inhibitory. Efflux was not energy dependent. Efflux is partially blocked by mercuric ions and completely blocked by an external pH of 9 to 11. Although efflux rates increased continuously with lowering of the pH, influx rates have a sharp maximum at pH 7.
机译：利用抗生素的荧光特性并测量[7-3H]四环素的吸收量，研究了光养细菌球形红球菌的四环素积累。积累是由载体介导的，Km约为300微米。外排似乎也是由媒介介导的，Km为25 mM。氯四环素竞争性地抑制四环素的转运。运输是依赖能量的。外流在外流过程中发生，当外流平衡外流时达到能量需求的稳态。运输被代谢抑制剂如抗霉素A，氰化物和碘乙酸盐抑制。质子导体如羰基氰化物间氯苯基具有很强的抑制作用。外排与能量无关。外排被汞离子部分阻止，并完全被9至11的外部pH阻止。尽管外排率随着pH的降低而持续增加，但在pH 7时，流入量却有一个急剧的最大值。

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